MBD5 Protein (AA 1-1498) (His tag)
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- Target See all MBD5 Proteins
- MBD5 (Methyl-CpG Binding Domain Protein 5 (MBD5))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1498
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Origin
- Mouse
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Source
- Insect Cells
- Purification tag / Conjugate
- This MBD5 protein is labelled with His tag.
- Application
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Sequence
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MNGGKECDGG DKEGGLAAIQ VPVGWQRRVD HNGVLYISPS GSLLSCLDQV KTYLLTDGTC KCGLECPLIL PKVFNFDPGA AVKQRTAEDV KADDDVTKLC IHKRKIIAVA TLHQSMEAPH PSLVLTSPGG GTNATPVVPS RAATPRSVRN KSHEGITNSV MPECKNPFKL MTGSSNAMGR LYMQDLPGSQ QQELHPVYPR QRLGSSEHGQ KSPFRGSHGG LPSPASSGSQ IYGDGSISPR TDPLGSPDVF TRNNPGFHGA PNSSPIHLNR TPLSPPSVML HGSPVQSSCA MAGRTNIPLS PTLTTKSPVM KKPMCNFSTN MEIPRAMFHH KPPQGPPPPP PPSCALQKKP LTSEKDPLGI LDPIPSKPVN QNPIIINPTS FHSNVHSQVP VMNVSMPPAV VPLPSNLPLP TVKPGHMNHG SHVQRIQHSA STSLSPSPVT SPVHMMGTGI GRIEASPQRS RSSSTSSDHG NFMMPPVGPQ ATCSGIKVPP RSPRSTIGSP RPSMPSSPST KSDGHHQYKD IPNPLIAGMS NVLNTPSSAA FPTAPAGNGS VKSQPGLLGM PLNQILNQHN AASFPASSLL SAAAKAQLAN QNKLAGNNSS SSNNSGAVAS SGNTEGHSTL NTMFPPTANM LLPTGEGQSG RAALRDKLMS QQKDSLRKRK QPPTTVLSLL RQSQMDSSAA PKPGPDLLRK HGQGSFPISS MSQLLQSMSC QSSHLSSNST PGCGGSNTAL PCSANQLHFP DPNMNSTVLQ NSLTQSIPLR GEAVHCHNAN TNFVHSNSPV PNHHLAGLIN QIQASGNCGM LSQSGMALGN SLHPNPPQSR ISTSSTPVIP NSIVSSYNQT SSEAGGSSLP SSIAIAGSNH PAITKTTSVL QDGVIVTTAA GNPLQSQLPI GSDFPFVGQE HALHFPSNST ANNHLPHPLN PSLLSSLPIS LPVNQQHLLN QNLLNILQPS AGEGDISSIN NSLNNHQLTH LQSLLNSNQM FPPNQQPQQH LLQGHQNLQA FQGQPTVPCP ANNNPMACLF QNFQVRMQGD AALLNKRIST QPGLTTLPEN PNLALPHFQD TPCELQPRID LGQPMKDGLV MGGQGDASVD AIYKAVVDAA SKGMQVVITT AVNSTTQISP IPALSAMSAF TASIGDPLNL SSAVSAVIHG RNMGGVDHDG RLRNARGARL PKNIDHGKNS SEGDGFECFK SASCHTSRKQ WDGEQSPRGE RNRWKYEEFL DHPGHIHSSP CHERPNNVST LPFLAGEQHP ILLPPRNCQG DKILEENFRY NNYKRTMMSF KERLESTVER CTHINGNRPR QSRGFGELLG TAKQDLVLEG QSPGSSNSLE SSLVKDYIHY NGDFNAKTIN GCVPSPSDAK SISSEDDLRN PDSPSSHELI HYRPRTFNVG DLVWGQIKGL TSWPGKFIRE DDVHNSCQQS PEEGKVEPEK LKTLTEGLEA YSRVRKRSRK SGKLNNHLEA AIHEAMSELD KMSGTVHQIP QGDRQMRPPK PKRRKISR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Mbd5 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product MBD5 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- MBD5 (Methyl-CpG Binding Domain Protein 5 (MBD5))
- Alternative Name
- Mbd5 (MBD5 Products)
- Synonyms
- MBD5 Protein, MRD1 Protein, 9430004D19Rik Protein, AA536666 Protein, AI426407 Protein, C030040A15Rik Protein, OTTMUSG00000012483 Protein, methyl-CpG binding domain protein 5 Protein, MBD5 Protein, mbd5 Protein, Mbd5 Protein
- Background
- Binds to heterochromatin. Does not interact with either methylated or unmethylated DNA (in vitro) (By similarity). {ECO:0000250}.
- Molecular Weight
- 161.2 kDa Including tag.
- UniProt
- B1AYB6
- Pathways
- Chromatin Binding
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