AKAP5 Protein (AA 1-745) (Strep Tag)

Catalog No. ABIN3131085

Product Details

Target: AKAP5 (A Kinase (PRKA) Anchor Protein 5 (AKAP5))

Protein Type: Recombinant

Protein Characteristics: AA 1-745

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This AKAP5 protein is labelled with Strep Tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS)

Sequence: METSVSEIQV ETKDEKGPVA ASPQKERQER KTATLCFKRR KKANKTKPKA GSRTAEETKK HTPEAGGSGQ RQPAGAWASI KGLVTHRKRS EPAKKQKPPE AEVQPEDGAL PKKKAKSRLK FPCLRFSRGA KRSRHSKLTE DSGYVRVQGE ADDLEIKAQT QPDDQAIQAG STQGLQEGVL VRDGKKSQES HISNSVTSGE NVIAIELELE NKSSAIQMGT PELEKETKVI TEKPSVQTQR ASLLESSAAG SPRSVTSAAP PSPATTHQHS LEEPSNGIRE SAPSGKDDRR KTAAEEKKSG ETALGQAEEA AVGQADKRAL SQAGEATAGH PEEATVIQAE SQAKEGKLSQ AEETTVAQAK ETVLSQAKEG ELSQAKKATV GQAEEATIDH TEKVTVDQAE ETTVGQAEEA TVGQAGEAIL SQAKEATVVG QAEEATVDRA EEATVGQAEE ATVGHTEKVT VDQAEEATVG QAEEATVGQA EEATVDWAEK PTVGQAEEAT VGQAEEATVG HTEKVTVDQA EEATVGQAEE ATVGHTEKVT VDHAEEATVG QAEEATVGQA EKVTVDHAEE ATVGQAEEAT VGQAEKVTVD HAEEATVGQA EEATVGQAEK VTVDQAEEPT VDQAEEAISS HAPDLKENGI DTEKPRSEES KRMEPIAIII TDTEISEFDV KKSKNVPKQF LISMENEQVG VFANDSDFEG RTSEQYETLL IETASSLVKN AIELSVEQLV NEMVSEDNQI NTLFQ
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: AKAP5 (A Kinase (PRKA) Anchor Protein 5 (AKAP5))

Alternative Name: Akap5 (AKAP5 Products)

Synonyms: AKAP5 Protein, AKAP75 Protein, AKAP79 Protein, H21 Protein, AKAP Protein, P75 Protein, 3526401B18Rik Protein, AKAP 150 Protein, AKAP-5 Protein, AKAP150 Protein, BB098886 Protein, Gm258 Protein, P150 Protein, Akap79 Protein, A kinase (PRKA) anchor protein 5 Protein, A-kinase anchoring protein 5 Protein, AKAP5 Protein, Akap5 Protein

Target Type: Viral Protein

Background: A-kinase anchor protein 5 (AKAP-5) (A-kinase anchor protein 150 kDa) (AKAP 150) (P150) (cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein),FUNCTION: Multivalent scaffold protein that anchors the cAMP-dependent protein kinase/PKA to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors. Association with the beta2-adrenergic receptor (beta2-AR) not only regulates beta2-AR signaling pathway, but also the activation by PKA by switching off the beta2-AR signaling cascade. Plays a role in long term synaptic potentiation by regulating protein trafficking from the dendritic recycling endosomes to the plasma membrane and controlling both structural and functional plasticity at excitatory synapses. {ECO:0000250|UniProtKB:P24588}.

Molecular Weight: 79.4 kDa

UniProt: D3YVF0

Pathways: cAMP Metabolic Process