FGA Protein (AA 37-789) (His tag)

Catalog No. ABIN3131128

Product Details

Target: FGA (Fibrinogen alpha Chain (FGA))

Protein Type: Recombinant

Protein Characteristics: AA 37-789

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This FGA protein is labelled with His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequence: GPRVVERHQS QCKDSDWPFC SDDDWNHKCP SGCRMKGLID EANQDFTNRI NKLKNSLFDF QRNNKDSNSL TRNIMEYLRG DFANANNFDN TYGQVSEDLR RRIEILRRKV IEKAQQIQAL QSNVRAQLID MKRLEVDIDI KIRSCKGSCS RAVNREINLQ DYEGHQKQLQ QVIAKELLPT KDRQYLPALK MSPVPDLVPG SFKSQLQEAP PEWKALTEMR QMRMELERPG KDGGSRGDSP GDSRGDSRGD FATRGPGSKA ENPTNPGPGG SGYWRPGNSG SGSDGNRNPG TTGSDGTGDW GTGSPRPGSD SGNFRPANPN WGVFSEFGDS SSPATRKEYH TGKAVTSKGD KELLIGKEKV TSSGTSTTHR SCSKTITKTV TGPDGRREVV KEVITSDDGS DCGDATELDI SHSFSGSLDE LSERHPDLSG FFDNHFGLIS PNFKEFGSKT HSDSDILTNI EDPSSHVPEF SSSSKTSTVK KQVTKTYKMA DEAGSEAHRE GETRNTKRGR ARARPTRDCD DVLQTQTSGA QNGIFSIKPP GSSKVFSVYC DQETSLGGWL LIQQRMDGSL NFNRTWQDYK RGFGSLNDKG EGEFWLGNDY LHLLTLRGSV LRVELEDWAG KEAYAEYHFR VGSEAEGYAL QVSSYRGTAG DALVQGSVEE GTEYTSHSNM QFSTFDRDAD QWEENCAEVY GGGWWYNSCQ AANLNGIYYP GGTYDPRNNS PYEIENGVVW VPFRGADYSL RAVRMKIRPL VGQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Fga Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: FGA (Fibrinogen alpha Chain (FGA))

Alternative Name: Fga (FGA Products)

Synonyms: Fib2 Protein, ENSMUSG00000059807 Protein, Fib Protein, Ac1873 Protein, Fba5e Protein, fibrinogen alpha chain Protein, FGA Protein, Fga Protein, LOC698244 Protein

Background: Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots (PubMed:7649481). In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization (PubMed:11389004). Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood (PubMed:7649481). However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo (PubMed:10930441). Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway (PubMed:19332769). Maternal fibrinogen is essential for successful pregnancy (PubMed:7649481). Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage (PubMed:12629066). May also facilitate the immune response via both innate and T-cell mediated pathways (PubMed:23487423). {ECO:0000250|UniProtKB:P02671, ECO:0000269|PubMed:10930441, ECO:0000269|PubMed:11389004, ECO:0000269|PubMed:12629066, ECO:0000269|PubMed:15972474, ECO:0000269|PubMed:19332769, ECO:0000269|PubMed:23487423, ECO:0000269|PubMed:7649481}.

Molecular Weight: 84.5 kDa Including tag.

UniProt: E9PV24