IL16 Protein (AA 1-1322) (Strep Tag)

Catalog No. ABIN3131766

Product Details

Target: IL16 (Interleukin 16 (IL16))

Protein Type: Recombinant

Protein Characteristics: AA 1-1322

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This IL16 protein is labelled with Strep Tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Sequence: MEPHGHSGKS RKSTKFRSIS RSLILCNAKT SDDGSSPDEK YPDPFETSLC QGKEGFFHSS MQLADTFEAG LSNIPDLALA SDSAQLAAAG SDRGKHCRKM FFMKESSSTS SKEKSGKPEA QSSSFLFPKA CHQRTRSNST SVNPYSAGEI DFPMTKKSAA PTDRQPYSLC SNRKSLSQQL DYPILGTARP TRSLSTAQLG QLSGGLQASV ISNIVLMKGQ AKGLGFSIVG GKDSIYGPIG IYVKSIFAGG AAAADGRLQE GDEILELNGE SMAGLTHQDA LQKFKQAKKG LLTLTVRTRL TTPPSLCSHL SPPLCRSLSS STCGAQDSSP FSLESPASPA STAKPNYRIM VEVSLKKEAG VGLGIGLCSI PYFQCISGIF VHTLSPGSVA HLDGRLRCGD EIVEINDSPV HCLTLNEVYT ILSHCDPGPV PIIVSRHPDP QVSEQQLKEA VAQAVEGVKF GKDRHQWSLE GVKRLESSWH GRPTLEKERE KHSAPPHRRA QKIMVRSSSD SSYMSGSPGG SPCSAGAEPQ PSEREGSTHS PSLSPGEEQE PCPGVPSRPQ QESPPLPESL ERESHPPLRL KKSFEILVRK PTSSKPKPPP RKYFKNDSEP QKKLEEKEKV TDPSGHTLPT CSQETRELLP LLLQEDTAGR APCTAACCPG PAASTQTSSS TEGESRRSAS PETPASPGKH PLLKRQARMD YSFDITAEDP WVRISDCIKN LFSPIMSENH SHTPLQPNTS LGEEDGTQGC PEGGLSKMDA ANGAPRVYKS ADGSTVKKGP PVAPKPAWFR QSLKGLRNRA PDPRRPPEVA SAIQPTPVSR DPPGPQPQAS SSIRQRISSF ENFGSSQLPD RGVQRLSLQP SSGETTKFPG KQDGGRFSGL LGQGATVTAK HRQTEVESMS TTFPNSSEVR DPGLPESPPP GQRPSTKALS PDPLLRLLTT QSEDTQGPGL KMPSQRARSF PLTRTQSCET KLLDEKASKL YSISSQLSSA VMKSLLCLPS SVSCGQITCI PKERVSPKSP CNNSSAAEGF GEAMASDTGF SLNLSELREY SEGLTEPGET EDRNHCSSQA GQSVISLLSA EELEKLIEEV RVLDEATLKQ LDSIHVTILH KEEGAGLGFS LAGGADLENK VITVHRVFPN GLASQEGTIQ KGNEVLSING KSLKGATHND ALAILRQARD PRQAVIVTRR TTVEATHDLN SSTDSAASAS AASDISVESK EATVCTVTLE KTSAGLGFSL EGGKGSLHGD KPLTINRIFK GTEQGEMVQP GDEILQLAGT AVQGLTRFEA WNVIKALPDG PVTIVIRRTS LQCKQTTASA DS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: IL16 (Interleukin 16 (IL16))

Alternative Name: Il16 (IL16 Products)

Synonyms: IL16 Protein, LCF Protein, NIL16 Protein, PRIL16 Protein, prIL-16 Protein, mKIAA4048 Protein, IL-16 Protein, interleukin 16 Protein, IL16 Protein, Il16 Protein

Background: Pro-interleukin-16 [Cleaved into: Interleukin-16 (IL-16) (Lymphocyte chemoattractant factor) (LCF)],FUNCTION: Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4., FUNCTION: Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane., FUNCTION: Isoform 2 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.

Molecular Weight: 141.4 kDa

UniProt: O54824