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SOCS5 Protein (AA 1-536) (His tag)

Crystallography grade SOCS5 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3131807
  • Target See all SOCS5 Proteins
    SOCS5 (Suppressor of Cytokine Signaling 5 (SOCS5))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-536
    Origin
    Mouse
    Source
    • 2
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This SOCS5 protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    MDKVGKMWNN LKYRCQNLFS HEGGSRNENV EMNPNRCPSV KEKSISLGEA APQQESSPLR ENVALQLGLS PSKTFSRRNQ NCAAEIPQVV EISIEKDSDS GATPGTRLAR RDSYSRHAPW GGKKKHSCST KTQSSLDTEK KFGRTRSGLQ RRERRYGVSS MQDMDSVSSR AVGSRSLRQR LQDTVGLCFP MRTYSKQSKP LFSNKRKIHL SELMLEKCPF PAGSDLAQKW HLIKQHTAPV SPHSTFFDTF DPSLVSTEDE EDRLRERRRL SIEEGVDPPP NAQIHTFEAT AQVNPLYKLG PKLAPGMTEI SGDGSAIPQT NCDSEEDSTT LCLQSRRQKQ RQVSGDSHAH VSRQGAWKVH TQIDYIHCLV PDLLQITGNP CYWGVMDRYE AEALLEGKPE GTFLLRDSAQ EDYLFSVSFR RYNRSLHARI EQWNHNFSFD AHDPCVFHSS TVTGLLEHYK DPSSCMFFEP LLTISLNRTF PFSLQYICRA VICRCTTYDG IDGLPLPSML QDFLKEYHYK QKVRVRWLER EPVKAK
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Socs5 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product SOCS5 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    SOCS5 (Suppressor of Cytokine Signaling 5 (SOCS5))
    Alternative Name
    Socs5 (SOCS5 Products)
    Synonyms
    SOCS5 Protein, cis6 Protein, cish5 Protein, cish6 Protein, socs-5 Protein, GB18949 Protein, socs5 Protein, zgc:153506 Protein, CIS6 Protein, CISH6 Protein, Cish5 Protein, SOCS-5 Protein, 1810018L08Rik Protein, mKIAA0671 Protein, RGD1564914 Protein, suppressor of cytokine signaling 5 Protein, suppressor of cytokine signaling 5a Protein, suppressor of cytokine signaling 5 L homeolog Protein, SOCS5 Protein, socs5 Protein, LOC552036 Protein, socs5a Protein, Tsp_07018 Protein, socs5.L Protein, Socs5 Protein
    Background
    SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Inhibits for instance EGF signaling by mediating the degradation of the EGF receptor/EGFR. Involved in the regulation of T-helper cell differentiation by inhibiting of the IL4 signaling pathway which promotes differentiation into the Th2 phenotype. Can also partially inhibit IL6 and LIF signaling. {ECO:0000269|PubMed:12242343}.
    Molecular Weight
    62.0 kDa Including tag.
    UniProt
    O54928
    Pathways
    JAK-STAT Signaling, EGFR Signaling Pathway, Positive Regulation of Immune Effector Process
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