SUPT5H Protein (AA 1-1082) (Strep Tag)

Catalog No. ABIN3131899

This Recombinant SUPT5H protein is produced in Cell-free protein synthesis (CFPS).

Quick Overview for SUPT5H Protein (AA 1-1082) (Strep Tag) (ABIN3131899)

Target: SUPT5H (Transcription Elongation Factor SPT5 (SUPT5H))

Protein Type: Recombinant

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1082

Purification tag / Conjugate: This SUPT5H protein is labelled with Strep Tag.

Sequence: MSDSEDSNFS EEEDSERSSE AEEAEVEEDQ RSAAGSEKEE EPEEEEEEEE EYDEEEEEED DDRPPKKPRH GGFILDEADV DDEYEDEDQW EDGAEDILEK EEIEASNIDN VVLDEDRSGA RRLQNLWRDQ REEELGEYYM KKYAKSSVGE TVYGGSDELS DDITQQQLLP GVKDPNLWTV KCKIGEERAT AISLMRKFIA YQFTDTPLQI KSVVAPEHVK GYIYVEAYKQ THVKQAIEGV GNLRLGYWNQ QMVPIKEMTD VLKVVKEVAN LKPKSWVRLK RGIYKDDIAQ VDYVEPSQNT ISLKMIPRID YDRIKARMSL KDWFAKRKKF KRPPQRLFDA EKIRSLGGDV ASDGDFLIFE GNRYSRKGFL FKSFAMSAVI TEGVKPTLSE LEKFEDQPEG IDLEVVTEST GKEREHNFQP GDNVEVCEGE LINLQGKVLS VDGNKITIMP KHEDLKDMLE FPAQELRKYF KMGDHVKVIA GRFEGDTGLI VRVEENFVIL FSDLTMHELK VLPRDLQLCS ETASGVDVGG QHEWGELVQL DPRTVGVIVR LERETFQVLN MHGKVVTVRH QAVTQKKDNR FAVALDSDQN NIHVKDIVKV IDGPHSGREG EIRHLYRSFA FLHCKKLVEN GGMFVCKARH LVLAGGSKPR DVTNLTVGGF TPMSPRISSP MHPSAEGQHG GFGSPGGMSR GRGRRDNELI GQTVRISQGP YKGYIGVVKD ATESTARVEL HSTCQTISVD RQRLTTVDSQ RPGGMTSTYG RTPMYGSQTP MYGSGSRTPM YGSQTPLQDG SRTPHYGSQT PLHDGSRTPA QSGAWDPNNP NTPSRAEEEY EYAFDDEPTP SPQAYGGTPN PQTPGYPDPS SPQVNPQYNP QTPGTPAMYN TDQFSPYAAP SPQGSYQPSP SPQSYHQVAP SPAGYQNTHS PASYHPTPSP MAYQASPSPS PVGYSPMTPG APSPGGYNPH TPGSGIEQNS SDWVTTDIQV KVRDTYLDTQ IVGQTGVIRS VTGGMCSVYL KDSEKVVSIS SEHLEPITPT KNNKVKVILG EDREATGVLL SIDGEDGIIR MDLEDQQIKI LNLRFLGKLL EA
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SUPT5H (Transcription Elongation Factor SPT5 (SUPT5H))

Alternative Name: Supt5h

Background: Transcription elongation factor SPT5 (DRB sensitivity-inducing factor large subunit) (DSIF large subunit),FUNCTION: Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites (By similarity). {ECO:0000250}.

Molecular Weight: 120.7 kDa

UniProt: O55201