Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

C4B Protein (AA 1448-1738) (His tag)

Crystallography grade C4b Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Catalog No. ABIN3132371
  • Target See all C4B (C4b) Proteins
    C4B (C4b) (Complement Component C4b (C4b))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1448-1738
    Origin
    • 14
    • 5
    • 2
    Mouse
    Source
    • 8
    • 8
    • 3
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This C4B protein is labelled with His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequence
    EAPKVVEEQE SRVQYTVCIW RNGKLGLSGM AIADITLLSG FHALRADLEK LTSLSDRYVS HFETDGPHVL LYFDSVPTTR ECVGFGASQE VVVGLVQPSS AVLYDYYSPD HKCSVFYAAP TKSQLLATLC SGDVCQCAEG KCPRLLRSLE RRVEDKDGYR MRFACYYPRV EYGFTVKVLR EDGRAAFRLF ESKITQVLHF RKDTMASIGQ TRNFLSRASC RLRLEPNKEY LIMGMDGETS DNKGDPQYLL DSNTWIEEMP SEQMCKSTRH RAACFQLKDF LMEFSSRGCQ V
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse C4b Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product C4b Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    C4B (C4b) (Complement Component C4b (C4b))
    Alternative Name
    C4b (C4b Products)
    Synonyms
    C4B1 Protein, C4B12 Protein, C4B2 Protein, C4B3 Protein, C4B5 Protein, C4BD Protein, C4B_2 Protein, C4F Protein, CH Protein, CO4 Protein, CPAMD3 Protein, C4 Protein, C4A2 Protein, C4A3 Protein, C4A4 Protein, C4A6 Protein, C4AD Protein, C4S Protein, CPAMD2 Protein, RG Protein, wu:fi14a03 Protein, C4B Protein, DKFZP469I0332 Protein, Slp Protein, Ss Protein, C4-2 Protein, C4l Protein, complement C4B (Chido blood group) Protein, complement C4A (Rodgers blood group) Protein, complement component 4 Protein, complement C4-B Protein, complement component 4B (Chido blood group) Protein, complement component 4A (Rodgers blood group) Protein, C4B Protein, C4A Protein, c4 Protein, LOC462577 Protein, C4a Protein, C4b Protein
    Background
    Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. Catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens (By similarity). {ECO:0000250}.
    Molecular Weight
    34.0 kDa Including tag.
    UniProt
    P01029
    Pathways
    Complement System
You are here:
Support