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EGF Protein (AA 29-1038) (His tag)

Crystallography grade EGF Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Catalog No. ABIN3132378
  • Target See all EGF Proteins
    EGF (Epidermal Growth Factor (EGF))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 29-1038
    Origin
    • 32
    • 18
    • 10
    • 4
    • 3
    • 1
    Mouse
    Source
    • 37
    • 9
    • 7
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This EGF protein is labelled with His tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Sequence
    WQTGNCQPGP LERSERSGTC AGPAPFLVFS QGKSISRIDP DGTNHQQLVV DAGISADMDI HYKKERLYWV DVERQVLLRV FLNGTGLEKV CNVERKVSGL AIDWIDDEVL WVDQQNGVIT VTDMTGKNSR VLLSSLKHPS NIAVDPIERL MFWSSEVTGS LHRAHLKGVD VKTLLETGGI SVLTLDVLDK RLFWVQDSGE GSHAYIHSCD YEGGSVRLIR HQARHSLSSM AFFGDRIFYS VLKSKAIWIA NKHTGKDTVR INLHPSFVTP GKLMVVHPRA QPRTEDAAKD PDPELLKQRG RPCRFGLCER DPKSHSSACA EGYTLSRDRK YCEDVNECAT QNHGCTLGCE NTPGSYHCTC PTGFVLLPDG KQCHELVSCP GNVSKCSHGC VLTSDGPRCI CPAGSVLGRD GKTCTGCSSP DNGGCSQICL PLRPGSWECD CFPGYDLQSD RKSCAASGPQ PLLLFANSQD IRHMHFDGTD YKVLLSRQMG MVFALDYDPV ESKIYFAQTA LKWIERANMD GSQRERLITE GVDTLEGLAL DWIGRRIYWT DSGKSVVGGS DLSGKHHRII IQERISRPRG IAVHPRARRL FWTDVGMSPR IESASLQGSD RVLIASSNLL EPSGITIDYL TDTLYWCDTK RSVIEMANLD GSKRRRLIQN DVGHPFSLAV FEDHLWVSDW AIPSVIRVNK RTGQNRVRLQ GSMLKPSSLV VVHPLAKPGA DPCLYRNGGC EHICQESLGT ARCLCREGFV KAWDGKMCLP QDYPILSGEN ADLSKEVTSL SNSTQAEVPD DDGTESSTLV AEIMVSGMNY EDDCGPGGCG SHARCVSDGE TAECQCLKGF ARDGNLCSDI DECVLARSDC PSTSSRCINT EGGYVCRCSE GYEGDGISCF DIDECQRGAH NCGENAACTN TEGGYNCTCA GRPSSPGLSC PDSTAPSLLG EDGHHLDRNS YPGCPSSYDG YCLNGGVCMH IESLDSYTCN CVIGYSGDRC QTRDLRWWEL RHAGYGQKHD
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Egf Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product EGF Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    EGF (Epidermal Growth Factor (EGF))
    Alternative Name
    Egf (EGF Products)
    Synonyms
    HOMG4 Protein, URG Protein, AI790464 Protein, CEGF Protein, epidermal growth factor Protein, pro-epidermal growth factor Protein, EGF Protein, egf Protein, CpipJ_CPIJ020278 Protein, Egf Protein
    Background
    EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity). {ECO:0000250}.
    Molecular Weight
    111.8 kDa Including tag.
    UniProt
    P01132
    Pathways
    NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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