Complement Factor H Protein (CFH) (AA 19-1234) (His tag)

Catalog No. ABIN3132582

Product Details

Target: Complement Factor H (CFH)

Protein Type: Recombinant

Protein Characteristics: AA 19-1234

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This Complement Factor H protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: EDCKGPPPRE NSEILSGSWS EQLYPEGTQA TYKCRPGYRT LGTIVKVCKN GKWVASNPSR ICRKKPCGHP GDTPFGSFRL AVGSQFEFGA KVVYTCDDGY QLLGEIDYRE CGADGWINDI PLCEVVKCLP VTELENGRIV SGAAETDQEY YFGQVVRFEC NSGFKIEGHK EIHCSENGLW SNEKPRCVEI LCTPPRVENG DGINVKPVYK ENERYHYKCK HGYVPKERGD AVCTGSGWSS QPFCEEKRCS PPYILNGIYT PHRIIHRSDD EIRYECNYGF YPVTGSTVSK CTPTGWIPVP RCTLKPCEFP QFKYGRLYYE ESLRPNFPVS IGNKYSYKCD NGFSPPSGYS WDYLRCTAQG WEPEVPCVRK CVFHYVENGD SAYWEKVYVQ GQSLKVQCYN GYSLQNGQDT MTCTENGWSP PPKCIRIKTC SASDIHIDNG FLSESSSIYA LNRETSYRCK QGYVTNTGEI SGSITCLQNG WSPQPSCIKS CDMPVFENSI TKNTRTWFKL NDKLDYECLV GFENEYKHTK GSITCTYYGW SDTPSCYERE CSVPTLDRKL VVSPRKEKYR VGDLLEFSCH SGHRVGPDSV QCYHFGWSPG FPTCKGQVAS CAPPLEILNG EINGAKKVEY SHGEVVKYDC KPRFLLKGPN KIQCVDGNWT TLPVCIEEER TCGDIPELEH GSAKCSVPPY HHGDSVEFIC EENFTMIGHG SVSCISGKWT QLPKCVATDQ LEKCRVLKST GIEAIKPKLT EFTHNSTMDY KCRDKQEYER SICINGKWDP EPNCTSKTSC PPPPQIPNTQ VIETTVKYLD GEKLSVLCQD NYLTQDSEEM VCKDGRWQSL PRCIEKIPCS QPPTIEHGSI NLPRSSEERR DSIESSSHEH GTTFSYVCDD GFRIPEENRI TCYMGKWSTP PRCVGLPCGP PPSIPLGTVS LELESYQHGE EVTYHCSTGF GIDGPAFIIC EGGKWSDPPK CIKTDCDVLP TVKNAIIRGK SKKSYRTGEQ VTFRCQSPYQ MNGSDTVTCV NSRWIGQPVC KDNSCVDPPH VPNATIVTRT KNKYLHGDRV RYECNKPLEL FGQVEVMCEN GIWTEKPKCR DSTGKCGPPP PIDNGDITSL SLPVYEPLSS VEYQCQKYYL LKGKKTITCR NGKWSEPPTC LHACVIPENI MESHNIILKW RHTEKIYSHS GEDIEFGCKY GYYKARDSPP FRTKCINGTI NYPTCV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Cfh Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Complement Factor H (CFH)

Alternative Name: Cfh (CFH Products)

Synonyms: AHUS1 Protein, AMBP1 Protein, ARMD4 Protein, ARMS1 Protein, CFHL3 Protein, FH Protein, FHL1 Protein, HF Protein, HF1 Protein, HF2 Protein, HUS Protein, Mud-1 Protein, NOM Protein, Sas-1 Protein, Sas1 Protein, Fh Protein, CFH Protein, im:7147412 Protein, si:ch211-207o17.2 Protein, fH Protein, armd4 Protein, arms1 Protein, cfh Protein, cfhl3 Protein, fhl1 Protein, hf1 Protein, hf2 Protein, hus Protein, complement factor H Protein, complement component factor h Protein, complement factor H-related protein 5 Protein, complement factor H L homeolog Protein, CFH Protein, Cfh Protein, cfh Protein, LOC100399888 Protein, LOC100733902 Protein, cfh.L Protein

Background: Factor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b)NBB complex (C5 convertase) in the alternative complement pathway. {ECO:0000250}.

Molecular Weight: 138.0 kDa Including tag.

UniProt: P06909

Pathways: Complement System, Cellular Response to Molecule of Bacterial Origin