CHMP6 Protein (AA 2-200) (His tag)

Catalog No. ABIN3132717

Product Details

Target: CHMP6 (Charged Multivesicular Body Protein 6 (CHMP6))

Protein Type: Recombinant

Protein Characteristics: AA 2-200

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This CHMP6 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequence: GNLFGRKKQS RVTEQDRAIL QLKQQRDKLR QYQKRVTQQL ERERALARQL LRDGRKERAK LLLKKKRYRE QLLDRTENQI SSLEAMVQSI EFTQIEMKVM EGLQVGNECL NKMHQVMSIE EVERILDETQ EAVEYQRQID ELLAGNFTQE DEDAILEELN AITQEQMELP EVPSEPLPDR NPEAPAKARS RQAELVAAS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Chmp6 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: CHMP6 (Charged Multivesicular Body Protein 6 (CHMP6))

Alternative Name: Chmp6 (CHMP6 Products)

Synonyms: chmp6 Protein, vps20 Protein, 2400004G01Rik Protein, VPS20 Protein, RGD1565325 Protein, chmp6l Protein, flj11749l Protein, wu:fb79d02 Protein, zgc:110067 Protein, charged multivesicular body protein 6 Protein, charged multivesicular body protein 6 L homeolog Protein, charged multivesicular body protein 6 S homeolog Protein, charged multivesicular body protein 6b Protein, LOC100280982 Protein, chmp6.L Protein, chmp6.S Protein, CHMP6 Protein, chmp6 Protein, Tsp_08316 Protein, Chmp6 Protein, chmp6b Protein

Background: Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. In the ESCRT-III complex, it probably serves as an acceptor for the ESCRT-II complex on endosomal membranes (By similarity). {ECO:0000250}.

Molecular Weight: 24.2 kDa Including tag.

UniProt: P0C0A3