AGT Protein (AA 25-477) (His tag)

Catalog No. ABIN3132878

Product Details

Target: AGT (Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT))

Protein Type: Recombinant

Protein Characteristics: AA 25-477

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This AGT protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: DRVYIHPFHL LYHNKSTCAQ LENPSVETLP ESTFEPVPIQ AKTSPVNEKT LHDQLVLAAE KLEDEDRKRA AQVAMIANFV GFRMYKMLNE AGSGASGAIL SPPALFGTLV SFYLGSLDPT ASQLQTLLDV PVKEGDCTSR LDGHKVLAAL RAVQGLLVTQ GGSSSQTPLL QSIMVGLFTA PGFRLKHSFV QSLALFTPAL FPRSLDLSTD PVLATEKINR FIKAVTGWKM NLPLEGVSTD STLLFNTYVH FQGTMRGFSQ LPGVHEFWVD NSISVSVPMI SGTGNFQHWS DAQNNFSVTC VPLGERATLL LIQPHCTSDL DRVEALIFRN DLLTWIENPP PRAIRLTLPQ LEIRGSYNLQ DLLAEDKLPT LLGAEANLSN IGDTNPRVGE VLNSILLELK AGEEEQPTTS VQQPGSPEAL DVTLSSPFLF AIYEQDSGTL HFLGRVNNPQ SVV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Agt Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: AGT (Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT))

Alternative Name: Agt (AGT Products)

Synonyms: ANHU Protein, SERPINA8 Protein, AI265500 Protein, AngI Protein, AngII Protein, Aogen Protein, Serpina8 Protein, ANRT Protein, Ang Protein, PAT Protein, wu:fb62f06 Protein, wu:fj87b02 Protein, zgc:111892 Protein, AGT Protein, angt Protein, ANGT Protein, angiotensinogen Protein, angiotensinogen (serpin peptidase inhibitor, clade A, member 8) Protein, AGT Protein, Agt Protein, agt Protein

Background: Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis., Angiotensin-2: acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone. {ECO:0000250}., Angiotensin-3: stimulates aldosterone release. {ECO:0000250}., Angiotensin 1-7: is a ligand for the G-protein coupled receptor MAS1. Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets.

Molecular Weight: 50.5 kDa Including tag.

UniProt: P11859

Pathways: JAK-STAT Signaling, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Monocarboxylic Acid Catabolic Process, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Regulation of long-term Neuronal Synaptic Plasticity