GPD1 Protein (AA 1-349) (His tag)
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- Target See all GPD1 Proteins
- GPD1 (Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-349
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Origin
- Mouse
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Source
- Insect Cells
- Purification tag / Conjugate
- This GPD1 protein is labelled with His tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
- Sequence
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MAGKKVCIVG SGNWGSAIAK IVGSNAGRLA HFDPRVTMWV FEEDIGGRKL TEIINTQHEN VKYLPGHKLP PNVVAIPDVV QAATGADILV FVVPHQFIGK ICDQLKGHLK ANTIGISLIK GVDEGPNGLK LISEVIGERL GIPMSVLMGA NIASEVAEEK FCETTIGCKD PAQGQLLKDL MQTPNFRITV VQEVDTVEIC GALKNIVAVG AGFCDGLGFG DNTKAAVIRL GLMEMIAFAK LFCSGTVSSA TFLESCGVAD LITTCYGGRN RKVAEAFART GKSIEQLEKE MLNGQKLQGP QTARELHSIL QHKGLVDKFP LFTAVYKVCY EGQPVGEFIR CLQNHPEHMHHHHHH
C-terminal His-tag - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Gpd1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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The protein is purified from the cleared cell lysate using EDTA and DTT resistant His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE.
Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot. - Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- in solution (20 mM Hepes, pH 7.4, 50 mM NaCl, 5 % Glycerin)
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- GPD1 (Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1))
- Alternative Name
- Gpd1 (GPD1 Products)
- Synonyms
- GPDH Protein, Gpd3 Protein, GPD-C Protein, GPDH-C Protein, HTGTI Protein, CG9042 Protein, DROGPDHA Protein, DmG3PDH Protein, Dmel\\CG9042 Protein, G3PDH Protein, G3pdh Protein, GAPDH Protein, GPD Protein, GPDA Protein, GPDH-1 Protein, Gdh Protein, Gpd Protein, alpha-GPD Protein, alpha-GPDH Protein, alpha-GPDH-1 Protein, alpha-Gpdh Protein, alphaGPDH Protein, alphaGpd Protein, alphaGpdh Protein, alphaGpdh-1 Protein, gpdh Protein, gpdh-1 Protein, sn-Gpdh Protein, AI747587 Protein, Gdc-1 Protein, Gdc1 Protein, mKIAA4010 Protein, gpd1 Protein, wu:fc30a07 Protein, zgc:63859 Protein, MGC79676 Protein, GPD1 Protein, LG3P Protein, zgc:112197 Protein, Gpd1 Protein, gpd1h Protein, gpd1l Protein, wu:fc58b05 Protein, zgc:66051 Protein, zgc:85742 Protein, glycerol-3-phosphate dehydrogenase 1 Protein, Glycerol-3-phosphate dehydrogenase [NAD(+)] Protein, glycerol-3-phosphate dehydrogenase Protein, Glycerol 3 phosphate dehydrogenase Protein, glycerol-3-phosphate dehydrogenase 1 (soluble) Protein, glycerol-3-phosphate dehydrogenase 1 L homeolog Protein, glycerol-3-phosphate dehydrogenase 1c Protein, glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic Protein, glycerol-3-phosphate dehydrogenase 1a Protein, glycerol-3-phosphate dehydrogenase 1b Protein, Gpd1 Protein, gpdh-1 Protein, GPD1 Protein, Tb11.02.5280 Protein, Gpdh Protein, gpd1.L Protein, gpd1c Protein, gpd1 Protein, LOC101071719 Protein, gpd1a Protein, gpd1b Protein
- Molecular Weight
- 38.5 kDa Including tag.
- UniProt
- P13707
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