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MECOM Protein (AA 1-1042) (His tag)

Crystallography grade MECOM Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Catalog No. ABIN3132979
  • Target See all MECOM Proteins
    MECOM (MDS1 and EVI1 Complex Locus (MECOM))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-1042
    Origin
    • 6
    • 1
    Mouse
    Source
    • 3
    • 3
    • 1
    Insect Cells
    Purification tag / Conjugate
    This MECOM protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
    Sequence
    MKSEEDPHEP MAPDIHEERQ HRCEDCDQLF ESKAELADHQ KFPCSTPHSA FSMVEEDLQQ NLESESDLRE IHGNQDCKEC DRVFPDLQSL EKHMLSHTEE REYKCDQCPK AFNWKSNLIR HQMSHDSGKH YECENCAKVF TDPSNLQRHI RSQHVGARAH ACPECGKTFA TSSGLKQHKH IHSSVKPFIC EVCHKSYTQF SNLCRHKRMH ADCRTQIKCK DCGQMFSTTS SLNKHRRFCE GKNHFAAGGF FGQGISLPGT PAMDKTSMVN MSHANPGLAD YFGTNRHPAG LTFPTAPGFS FSFPGLFPSG LYHRPPLIPA SPPVKGLSST EQSNKCQSPL LTHPQILPAT QDILKALSKH PPVGDNKPVE LLPERSSEER PLEKISDQSE SSDLDDVSTP SGSDLETTSG SDLESDLESD KEKCKENGKM FKDKVSPLQN LASITNKKEH NNHSVFSASV EEQSAVSGAV NDSIKAIASI AEKYFGSTGL VGLQDKKVGA LPYPSMFPLP FFPAFSQSMY PFPDRDLRSL PLKMEPQSPS EVKKLQKGSS ESPFDLTTKR KDEKPLTSGP SKPSGTPATS QDQPLDLSMG SRGRASGTKL TEPRKNHVFG EKKGSNMDTR PSSDGSLQHA RPTPFFMDPI YRVEKRKLTD PLEALKEKYL RPSPGFLFHP QMSAIENMAE KLESFSALKP EASELLQSVP SMFSFRAPPN TLPENLLRKG KERYTCRYCG KIFPRSANLT RHLRTHTGEQ PYRCKYCDRS FSISSNLQRH VRNIHNKEKP FKCHLCDRCF GQQTNLDRHL KKHENGNMSG TATSSPHSEL ESAGAILDDK EDAYFTEIRN FIGNSNHGSQ SPRNMEERMN GSHFKDKKAL ATSQNSDLLD DEEVEDEVLL DEEDEDNDIP GKPRKELGVT RLDEEIPEDD YEEAGALEMS CKASPVRYKE EDYKSGLSAL DHIRHFTDSL KMREMEENQY TDAELSSISS SHVPEELKQT LHRKSKSQAY AMMLSLSDKD SLHPTSHSSS NVWHSMARAA AESSAIQSIS HV
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Mecom Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product MECOM Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    MECOM (MDS1 and EVI1 Complex Locus (MECOM))
    Alternative Name
    Mecom (MECOM Products)
    Synonyms
    AML1-EVI-1 Protein, EVI1 Protein, MDS1 Protein, MDS1-EVI1 Protein, PRDM3 Protein, D630039M04Rik Protein, Evi-1 Protein, Evi1 Protein, Jbo Protein, Mds Protein, Mds1 Protein, Mds1-Evi1 Protein, Prdm3 Protein, Znfpr1b1 Protein, evi1 Protein, im:7140716 Protein, prdm3 Protein, evi-1 Protein, mecom Protein, mecom-a Protein, mecom-b Protein, xEvi-1 Protein, evi1-B Protein, MDS1 and EVI1 complex locus Protein, MDS1 and EVI1 complex locus L homeolog Protein, MDS1 and EVI1 complex locus S homeolog Protein, MECOM Protein, Mecom Protein, mecom Protein, mecom.L Protein, mecom.S Protein
    Background
    Functions as a transcriptional regulator binding to DNA sequences in the promoter region of target genes and regulating positively or negatively their expression. Oncogene which plays a role in development, cell proliferation and differentiation. May also play a role in apoptosis through regulation of the JNK and TGF-beta signaling. Involved in hematopoiesis. {ECO:0000269|PubMed:15889140, ECO:0000269|PubMed:18619962, ECO:0000269|PubMed:19767769}.
    Molecular Weight
    117.8 kDa Including tag.
    UniProt
    P14404
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