ABCB4 Protein (AA 352-708) (His tag)

Catalog No. ABIN3133294

Product Details

Target: ABCB4 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 4 (ABCB4))

Protein Type: Recombinant

Protein Characteristics: AA 352-708

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This ABCB4 protein is labelled with His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequence: DAFANARGAA YVIFDIIDNN PKIDSFSERG HKPDNIKGNL EFSDVHFSYP SRANIKILKG LNLKVKSGQT VALVGNSGCG KSTTVQLLQR LYDPTEGKIS IDGQDIRNFN VRCLREIIGV VSQEPVLFST TIAENIRYGR GNVTMDEIEK AVKEANAYDF IMKLPQKFDT LVGDRGAQLS GGQKQRIAIA RALVRNPKIL LLDEATSALD TESEAEVQAA LDKAREGRTT IVIAHRLSTI RNADVIAGFE DGVIVEQGSH SELMKKEGIY FRLVNMQTAG SQILSEEFEV ELSDEKAAGD VAPNGWKARI FRNSTKKSLK SPHQNRLDEE TNELDANVPP VSFLKVLKLN KTEWPYF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Abcb4 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ABCB4 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 4 (ABCB4))

Alternative Name: Abcb4 (ABCB4 Products)

Synonyms: ABC21 Protein, GBD1 Protein, ICP3 Protein, MDR2 Protein, MDR2/3 Protein, MDR3 Protein, PFIC-3 Protein, PGY3 Protein, zgc:172149 Protein, Mdr2 Protein, Pgy-2 Protein, Pgy2 Protein, mdr-2 Protein, Pgy3 Protein, ABCB4a Protein, DEFB1 Protein, PGP2 Protein, PGY2 Protein, PGP3 Protein, ABCB4 Protein, RUNDC3B Protein, ATP binding cassette subfamily B member 4 Protein, ATP-binding cassette, sub-family B (MDR/TAP), member 4 Protein, beta-defensin 1-like Protein, ATP-binding cassette, sub-family B (MDR/TAP), member 1B Protein, RUN domain-containing protein 3B Protein, ABCB4 Protein, abcb4 Protein, Abcb4 Protein, LOC100861170 Protein, Abcb1b Protein, LOC101122517 Protein

Background: Energy-dependent phospholipid efflux translocator that acts as a positive regulator of biliary lipid secretion. Functions as a floppase that translocates specifically phosphatidylcholine (PC) from the inner to the outer leaflet of the canalicular membrane bilayer into the canaliculi between hepatocytes. Translocation of PC makes the biliary phospholipids available for extraction into the canaliculi lumen by bile salt mixed micelles and therefore protects the biliary tree from the detergent activity of bile salts (PubMed:8106172, PubMed:7912658, PubMed:7592705, PubMed:7814632, PubMed:8725158, PubMed:9366571). Plays a role in the recruitment of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SM) molecules to nonraft membranes and to further enrichment of SM and cholesterol in raft membranes in hepatocytes (By similarity). Required for proper phospholipid bile formation (PubMed:8106172). Indirectly involved in cholesterol efflux activity from hepatocytes into the canalicular lumen in the presence of bile salts in an ATP-dependent manner (PubMed:7814632, PubMed:8725158). May promote biliary phospholipid secretion as canaliculi-containing vesicles from the canalicular plasma membrane (PubMed:9366571). In cooperation with ATP8B1, functions to protect hepatocytes from the deleterious detergent activity of bile salts (PubMed:21820390). Does not confer multidrug resistance (PubMed:1990275). {ECO:0000250|UniProtKB:P21439, ECO:0000269|PubMed:1990275, ECO:0000269|PubMed:21820390, ECO:0000269|PubMed:7592705, ECO:0000269|PubMed:7814632, ECO:0000269|PubMed:7912658, ECO:0000269|PubMed:8106172, ECO:0000269|PubMed:8725158, ECO:0000269|PubMed:9366571}.

Molecular Weight: 40.5 kDa Including tag.

UniProt: P21440

Pathways: Regulation of Lipid Metabolism by PPARalpha