gamma 1 Adaptin Protein (AA 2-822) (His tag)

Catalog No. ABIN3133342

Product Details

Target: gamma 1 Adaptin (AP1G1) (Adaptor-Related Protein Complex 1, gamma 1 Subunit (AP1G1))

Protein Type: Recombinant

Protein Characteristics: AA 2-822

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This gamma 1 Adaptin protein is labelled with His tag.

Application: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: PAPIRLRELI RTIRTARTQA EEREMIQKEC AAIRSSFREE DNTYRCRNVA KLLYMHMLGY PAHFGQLECL KLIASQKFTD KRIGYLGAML LLDERQDVHL LMTNCIKNDL NHSTQFVQGL ALCTLGCMGS SEMCRDLAGE VEKLLKTSNS YLRKKAALCA VHVIRKVPEL MEMFLPATKN LLNEKNHGVL HTSVVLLTEM CERSPDMLAH FRKLVPQLVR ILKNLIMSGY SPEHDVSGIS DPFLQVRILR LLRILGRNDD DSSEAMNDIL AQVATNTETS KNVGNAILYE TVLTIMDIKS ESGLRVLAIN ILGRFLLNND KNIRYVALTS LLKTVQTDHN AVQRHRSTIV DCLKDLDVSI KRRAMELSFA LVNGNNIRGM MKELLYFLDS CEPEFKADCA SGIFLAAEKY APSKRWHIDT IMRVLTTAGS YVRDDAVPNL IQLITNSVEM HAYTVQRLYK AILGDYSQQP LVQVAAWCIG EYGDLLVSGQ CEEEEPIQVT EDEVLDILES VLISNMSTSV TRGYALTAIM KLSTRFTCTV NRIKKVVSIY GSSIDVELQQ RAVEYNALFK KYDHMRSALL ERMPVMEKVT TNGPSEIVQT NGETEPAPLE TKPPPSGPQP TSQANDLLDL LGGNDITPVI PTAPTSKPAS AGGELLDLLG DITLTGAPAA APTPASVPQI SQPPFLLDGL SSQPLFNDIA PGIPSITAYS KNGLKIEFTF ERSNTNPSVT VITIQASNST ELDMTDFVFQ AAVPKTFQLQ LLSPSSSVVP AFNTGTITQV IKVLNPQKQQ LRMRIKLTYN HKGSAMQDLA EVNNFPPQSW Q
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Ap1g1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: gamma 1 Adaptin (AP1G1) (Adaptor-Related Protein Complex 1, gamma 1 Subunit (AP1G1))

Alternative Name: Ap1g1 (AP1G1 Products)

Synonyms: ADTG Protein, CLAPG1 Protein, AA409002 Protein, AU041323 Protein, AW551707 Protein, Adtg Protein, D8Ertd374e Protein, adtg Protein, wu:fc30a11 Protein, zgc:56079 Protein, AP1G1 Protein, adaptor related protein complex 1 gamma 1 subunit Protein, AP-1 complex subunit gamma-1 Protein, AP-1 adaptor complex gamma subunit Apl4 Protein, hypothetical protein Protein, adaptor protein complex AP-1, gamma 1 subunit Protein, adaptor-related protein complex 1, gamma 1 subunit Protein, adaptor related protein complex 1 gamma 1 subunit L homeolog Protein, gamma-adaptin Protein, AP1G1 Protein, NCU04121 Protein, PTRG_00081 Protein, SJAG_01809 Protein, UREG_02085 Protein, BDBG_07402 Protein, PAAG_11376 Protein, MCYG_00840 Protein, PITG_11143 Protein, VDBG_09354 Protein, Ap1g1 Protein, ap1g1 Protein, ap1g1.L Protein, ANI_1_330014 Protein, AOR_1_802184 Protein, MGYG_01130 Protein, EDI_324200 Protein, TERG_00390 Protein, Tsp_09544 Protein

Background: Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

Molecular Weight: 92.2 kDa Including tag.

UniProt: P22892