LTA4H Protein (AA 2-611) (His tag)

Catalog No. ABIN3133405

Product Details

Target: LTA4H (Leukotriene A4 Hydrolase (LTA4H))

Protein Type: Recombinant

Protein Characteristics: AA 2-611

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This LTA4H protein is labelled with His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequence: PEVADTCSLA SPASVCRTQH LHLRCSVDFA RRTLTGTAAL TVQSQEENLR SLTLDTKDLT IEKVVINGQE VKYTLGESQG YKGSPMEISL PIALSKNQEI VIEISFETSP KSSALQWLTP EQTSGKQHPY LFSQCQAIHC RAILPCQDTP SVKLTYTAEV SVPKELVALM SAIRDGEAPD PEDPSRKIYR FNQRVPIPCY LIALVVGALE SRQIGPRTLV WSEKEQVEKS ANEFSETESM LKIAEDLGGP YVWGQYDLLV LPPSFPYGGM ENPCLTFVTP TLLAGDKSLS NVIAHEISHS WTGNLVTNKT WDHFWLNEGH TVYLERHICG RLFGEKFRHF HALGGWGELQ NTIKTFGESH PFTKLVVDLK DVDPDVAYSS IPYEKGFALL FYLEQLLGGP EVFLGFLKAY VKKFSYQSVT TDDWKSFLYS HFKDKVDLLN QVDWNTWLYA PGLPPVKPNY DVTLTNACIA LSQRWVTAKE EDLSSFSIAD LKDLSSHQLN EFLAQVLQKA PLPLGHIKRM QEVYNFNAIN NSEIRFRWLR LCIQSKWEEA IPLALKMATE QGRMKFTRPL FKDLAAFDKS HDQAVHTYQE HKASMHPVTA MLVGRDLKVD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Lta4h Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: LTA4H (Leukotriene A4 Hydrolase (LTA4H))

Alternative Name: Lta4h (LTA4H Products)

Synonyms: LTA4H Protein, zgc:85809 Protein, MGC78867 Protein, lta4h Protein, MGC69197 Protein, LOC732953 Protein, DDBDRAFT_0191291 Protein, DDBDRAFT_0216768 Protein, DDB_0191291 Protein, DDB_0216768 Protein, AmpT Protein, leukotriene A4 hydrolase Protein, leukotriene A4 hydrolase L homeolog Protein, Leukotriene A4 hydrolase Protein, LTA4H Protein, lta4h Protein, lta4h.L Protein, LOC732953 Protein, Shew185_1367 Protein, Sbal195_1406 Protein, XfasM23_0741 Protein, Sbal223_2977 Protein, lkhA Protein, Lta4h Protein

Background: Epoxide hydrolase that catalyzes the final step in the biosynthesis of the proinflammatory mediator leukotriene B4. Has also aminopeptidase activity. {ECO:0000269|PubMed:9287304}.

Molecular Weight: 69.9 kDa Including tag.

UniProt: P24527