EPS15 Protein (AA 2-897) (His tag)

Catalog No. ABIN3134030

Product Details

Target: EPS15 (Epidermal Growth Factor Receptor Pathway Substrate 15 (EPS15))

Protein Type: Recombinant

Protein Characteristics: AA 2-897

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This EPS15 protein is labelled with His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA

Sequence: AAAAQLSLTQ LSSGNPVYEK YYRQVEAGNT GRVLALDAAA FLKKSGLPDL ILGKIWDLAD TDGKGVLSKQ EFFVALRLVA CAQNGLEVSL SSLSLAVPPP RFHDSSSPLL TSGPSVAELP WAVKSEDKAK YDAIFDSLSP VDGFLSGDKV KPVLLNSKLP VEILGRVWEL SDIDHDGKLD RDEFAVAMFL VYCALEKEPV PMSLPPALVP PSKRKTWVVS PAEKAKYDEI FLKTDKDMDG YVSGLEVRET FLKTGLPSAL LAHIWSLCDT KGCGKLSKDQ FALAFHLINQ KLIKGIDPPH SLTPEMIPPS DRSSLQKNIT GSSPVADFSA IKELDTLNNE IVDLQREKNN VEQDLKEKED TVKQRTSEVQ DLQDEVQRES INLQKLQAQK QQVQELLGEL DEQKAQLEEQ LQEVRKKCAE EAQLISSLKA EITSQESQIS SYEEELLKAR EELSRLQQET AQLEESVESG KAQLEPLQQH LQESQQEISS MQMRLEMKDL ETDNNQSNWS SSPQSVLVNG ATDYCSLSTS SSETANFNEH AEGQNNLESE PTHQESSVRS SPEIAPSDVT DESEAVTVAG NEKVTPRFDD DKHSKEEDPF NVESSSLTDA VADTNLDFFQ SDPFVGSDPF KDDPFGKIDP FGGDPFKGSD PFASDCFFKQ TSTDPFTTSS TDPFSASSNS SNTSVETWKH NDPFAPGGTV VAAASDSATD PFASVFGNES FGDGFADFST LSKVNNEDAF NPTISSSTSS VTIAKPMLEE TASKSEDVPP ALPPKVGTPT RPCPPPPGKR PINKLDSSDP LKLNDPFQPF PGNDSPKEKD PDMFCDPFTS STTTNKEADP SNFANFSAYP SEEDMIEWAK RESEREEEQR LARLNQQEQE DLELAIALSK SEISEA
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Eps15 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: EPS15 (Epidermal Growth Factor Receptor Pathway Substrate 15 (EPS15))

Alternative Name: Eps15 (EPS15 Products)

Synonyms: AF-1P Protein, AF1P Protein, MLLT5 Protein, CG16932 Protein, Dmel\\CG16932 Protein, EH1 Protein, EPS-15 Protein, Eps15 Protein, anon-WO0118547.85 Protein, eps-15 Protein, eps15 Protein, i31 Protein, 2410112D09Rik Protein, RGD1307641 Protein, epidermal growth factor receptor pathway substrate 15 Protein, Epidermal growth factor receptor pathway substrate clone 15 Protein, hypothetical protein Protein, EPS15 Protein, Eps-15 Protein, eps15 Protein, Eps15 Protein, PGTG_06574 Protein

Background: Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR), internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.

Molecular Weight: 99.3 kDa Including tag.

UniProt: P42567

Pathways: EGFR Signaling Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, EGFR Downregulation