NEK1 Protein (AA 1-1203) (His tag)
-
- Target See all NEK1 Proteins
- NEK1 (NIMA-Related Kinase 1 (NEK1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1203
-
Origin
- Mouse
-
Source
- Insect Cells
- Purification tag / Conjugate
- This NEK1 protein is labelled with His tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
- Sequence
-
MEKYVRLQKI GEGSFGKAVL VKSTEDGRHY VIKEINISRM SDKERQESRR EVAVLANMKH PNIVQYKESF EENGSLYIVM DYCEGGDLFK RINAQKGALF QEDQILDWFV QICLALKHVH DRKILHRDIK SQNIFLTKDG TVQLGDFGIA RVLNSTVELA RTCIGTPYYL SPEICENKPY NNKSDIWALG CVLYELCTLK HAFEAGNMKN LVLKIISGSF PPVSPHYSYD LRSLLSQLFK RNPRDRPSVN SILEKGFIAK RIEKFLSPQL IAEEFCLKTL SKFGPQPLPG KRPASGQGVS SFVPAQKITK PAAKYGVPLT YKKYGDKKLL EKKPPPKHKQ AHQIPVKKMN SGEERKKMSE EAAKKRRLEF IEKEKKQKDQ IRFLKAEQMK RQEKQRLERI NRAREQGWRN VLRAGGSGEV KASFFGIGGA VSPSPCSPRG QYEHYHAIFD QMQRLRAEDN EARWKGGIYG RWLPERQKGH LAVERANQVE EFLQRKREAM QNKARAEGHV VYLARLRQIR LQNFNERQQI KAKLRGENKE ADGTKGQEAT EETDMRLKKM ESLKAQTNAR AAVLKEQLER KRKEAYEREK KVWEEHLVAR VKSSDVPLPL ELLETGGSPS KQQVKPVISV TSALKEVGLD GSLTDTQEEE MEKSNSAISS KREILRRLNE NLKAQEDEKE KQHHSGSCET VGHKDEREYE TENAISSDRK KWEMGGQLVI PLDAVTLDTS FSATEKHTVG EVIKLDSNGS PRKVWGKNPT DSVLKILGEA ELQLQTELLE NTSFKSEVYA EEENYKPLLT EEENLQCISK EINPSATVDS TETKSPKFTE VSPQMSEGNV EEPDDLETEV LQEPSSTHTD GSLPPVLNDV WTREKEAAKE TELEDKVAVQ QSEVCEDRIP GNVDQSCKDQ RDPAVDDSPQ SGCDVEKSVQ PESIFQKVVH SKDLNLVQAV HCSPEEPIPI RSHSDSPPKT KSKNSLLIGL STGLFDANNP KMLRTCSLPD LSKLFRTLMD VPTVGDVHQD SLEIDELEDE PIKEGPSDSE DTVFEETDTD LQELQASMEQ LLREQPGDEY SEEEESVLKS SDVEQTARGT DAPDEEDNPS SESALNEEWH SDNSDAETTS ECEYDSVFNH LEELRLHLEQ EMGFEKFFEV YEKVKAIHED EDENIEICST IVENILGNEH QHLYAKILHL VMADGAYQED NDE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
-
- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Nek1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
-
Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product NEK1 Protein
-
-
- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
-
Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
-
- Target
- NEK1 (NIMA-Related Kinase 1 (NEK1))
- Alternative Name
- Nek1 (NEK1 Products)
- Synonyms
- D8Ertd790e Protein, kat Protein, NY-REN-55 Protein, SRPS2 Protein, SRPS2A Protein, ATNEK1 Protein, F20D21.32 Protein, F20D21_32 Protein, NIMA-RELATED KINASE1 Protein, NIMA-related serine/threonine kinase 1 Protein, NIMA (never in mitosis gene a)-related expressed kinase 1 Protein, NIMA related kinase 1 Protein, NIMA-related kinase 1 Protein, NIMA-related serine/threonine kinase 1 Protein, Nek1 Protein, NEK1 Protein
- Background
- Phosphorylates serines and threonines, but also appears to possess tyrosine kinase activity. Implicated in the control of meiosis. Involved in cilium assembly. In response to injury that includes DNA damage, NEK1 phosphorylates VDAC1 to limit mitochondrial cell death (By similarity). {ECO:0000250}.
- Molecular Weight
- 137.6 kDa Including tag.
- UniProt
- P51954
-