POLR1C Protein (AA 2-346) (His tag)

Catalog No. ABIN3134426

Product Details

Target: POLR1C (Polymerase (RNA) I Polypeptide C, 30kDa (POLR1C))

Protein Type: Recombinant

Protein Characteristics: AA 2-346

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This POLR1C protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequence: AAAQAVEEMR TRVVLGEFGV RNVHTTDFPG NYAGYDDAWD QNRFEKNFRV DVVQMDEDTL EFDMVGIDAA IANAFRRILL AEVPTMAVEK VLVYNNTSIV QDEILAHRLG LIPILADPRL FEYRNQGEEE GTEIDTLQFR LQVRCTRNPN AAKDSSDPNE LYVNHKVYTR HMTWVPLGNQ ADVFPEGTIR PVHDDILIAQ LRPGQEIDLM MHCVKGIGKD HAKFSPVATA SYRLLPAITL LEPVEGEAAE ELSQCFSPGV IEVEEVQGKK VARVANARLD TFSREIFRHE KLKKAVRLAR VRDHYIFSVE STGVLPPDVL VSEAIKILMG KCRRFLDELD AVEMD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Polr1c Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: POLR1C (Polymerase (RNA) I Polypeptide C, 30kDa (POLR1C))

Alternative Name: Polr1c (POLR1C Products)

Synonyms: zgc:65973 Protein, rpa39 Protein, rpa40 Protein, rpa5 Protein, rpac1 Protein, POLR1C Protein, 40kDa Protein, AA409007 Protein, AA959927 Protein, AL024089 Protein, Polr1e Protein, RPA40 Protein, Rpo1-1 Protein, Ac2-127 Protein, RPA39 Protein, RPA5 Protein, RPAC1 Protein, TCS3 Protein, polymerase (RNA) I polypeptide C Protein, RNA polymerase I subunit C Protein, polymerase (RNA) I polypeptide C L homeolog Protein, polymerase (RNA) I polypeptide C, 30kDa Protein, polr1c Protein, POLR1C Protein, polr1c.L Protein, Polr1c Protein

Background: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity). {ECO:0000250|UniProtKB:O15160, ECO:0000250|UniProtKB:P07703}.

Molecular Weight: 39.9 kDa Including tag.

UniProt: P52432