DEPDC5 Protein (AA 1-1591) (Strep Tag)

Catalog No. ABIN3134576

Product Details

Target: DEPDC5 (DEP Domain Containing 5 (DEPDC5))

Protein Type: Recombinant

Protein Characteristics: AA 1-1591

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This DEPDC5 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MRTTKVYKLV IHKKGFGGSD DELVVNPKVF PHIKLGDIVE IAHPNDEYSP LLLQVKSLKE DLQKETISVD QTVTQVFRLR PYQDVYVNVV DPKDVTLDLV ELTFKDQYIG RGDMWRLKKS LVSTCAYITQ KVEFAGIRAQ AGELWVKNEK VMCGYISEET RVVFRSTSAM VYIFIQMSCE MWDFDIYGDL YFEKAVNGFL ADLFTKWKEK NCSHEVTVVL FSRTFYDAKS IDEFPEINRA SIQEDHKGRF YEDFYKVVVQ NERREEWTSL LVTIKKLFIQ YPVLVRLEQA GGFPQGDNST SAQGNYLEAI NLSFNVFDKH YINRNFDRTG QMSVVITPGV GVFEVDRLLM ILTKQRMIDN GIGVDLVCMG EQPLHAVPLF KLHNRSVPRD SRLGDDYNIP HWINHSFYTS KSQLFCNSFT PRIKLAGKKS ASEKTKNGRD TSLGTPKESE NTLPIQVDYD AYDAQVFRLP GPSRAQRLAT CRSVREQENH SRKSASSCDV SSSPSLPSRA LPTEEVRSQA SDDSSLGKST NILMIPNPHL HQYEVSSSLG YTSTRDVLEN MIEPPQRDSS APGRFHVGSA ESMLHVRPGG YTPQRALINP FAPSRMPMKL TSNRRRWMHT FPVGPSGEAI QIHHQTRQNM AELQGSRQRD PTHSSAELLE LAYHEAAGRH STSRQPGDSM SLNFSGTEEL SVSLLSNSST GVNPRTQNKD SLEDSVSTSP DPMPGFCCTV GVDWKSLTTP ACLPLTTDYF PDRQGLQNDY TEGCYDLLPE ADMDRRDEEG VQMTAQQVFE EFICQRLMQG YQIIVQPKTQ KPNTTVPPPL SSSPLYSRGL VSRNRPEEEG QYWLSMGRTF HKVTLKDKMI TVTRYLPKYP YESAQIHYTY SLCPSHSDSE FVSCWVDFCH ERLEEYKWNY LDQYICSAGS EDFSLIESLK FWRTRFLLLP ACVTATKRIT EGEVHCDIYG DKPRADEDEW QLLDGFIRFV EGLNRIRRRH RSDRMIRKGT AMKGLQMTGP ISAHSLEAAG PPVGKKGTSA LSALLEMEAS QKSLGEQQTT VHGKSSTQPA ENSSVAMTPT YVDSPRKDGA FFMEFVRSPR TASSAFYPQA SVDQTAPLVL DSTSLGVSTG QPMDRGNNQT FGNSQNIEQA FPSANSGDYS SQQHVASSLT SSSTLVEILE AMKHPSTGVQ LLSEQKGLSP CCFISAEVVH WLMNNVEGVQ TQAMGIDIMQ KMLEEQLITH ASGEAWRTFI YGFYFYKIVM DKEPERVAMQ QPSAPWYTAG ADDFASFQRK WFEVAFVAEE LVHSEIPAFL LPWLPSRPAS YASRHSSFSR SFGGRSQAAA LLAATVPEQR TVTLDVDVNN RTDRLEWCSC YYHGNFSLNA AFEIKLHWMA VTATVLFEMV QGWHRKATSC GFLLVPVLEG PFALPSYLYG DPLRAQLFIP LNLSCLLKEG SEHLFDSFEP ETYWDRMHLF QEAIAHRFGF VQDKYSVSAF NFPAENKPQY IHVTGTVFLQ LPYSKRKFSG QQRRRRNSTS STNQNMFCEE RVGYNWAYNT MLTKTWRSSA TGDEKFADRL LKDFTDFCIN RDNRLVTFWT NCLEKMHASA P
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: DEPDC5 (DEP Domain Containing 5 (DEPDC5))

Alternative Name: Depdc5 (DEPDC5 Products)

Synonyms: DEPDC5 Protein, DEP.5 Protein, FFEVF Protein, AV016528 Protein, DEP domain containing 5 Protein, DEP domain-containing protein 5 Protein, Depdc5 Protein, DEPDC5 Protein, LOC100434001 Protein, LOC100576879 Protein

Background: GATOR1 complex protein DEPDC5 (DEP domain-containing protein 5),FUNCTION: As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the mTORC1 pathway (PubMed:31548394). In response to amino acid depletion, the GATOR1 complex has GTPase activating protein (GAP) activity and strongly increases GTP hydrolysis by RagA/RRAGA (or RagB/RRAGB) within heterodimeric Rag complexes, thereby turning them into their inactive GDP-bound form, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling (By similarity). In the presence of abundant amino acids, the GATOR1 complex is negatively regulated by GATOR2, the other GATOR subcomplex, in this amino acid-sensing branch of the TORC1 pathway (By similarity). Within the GATOR1 complex, DEPDC5 mediates direct interaction with the nucleotide-binding pocket of small GTPases Rag (RagA/RRAGA, RagB/RRAGB, RagC/RRAGC and/or RagD/RRAGD) and coordinates their nucleotide loading states by promoting RagA/RRAGA or RagB/RRAGB into their GDP-binding state and RagC/RRAGC or RagD/RRAGD into their GTP-binding state (By similarity). However, it does not execute the GAP activity, which is mediated by NPRL2 (By similarity). {ECO:0000250|UniProtKB:O75140, ECO:0000269|PubMed:31548394}.

Molecular Weight: 180.4 kDa

UniProt: P61460