RPS3 Protein (AA 2-243) (His tag)

Catalog No. ABIN3134590

Product Details

Target: RPS3 (Ribosomal Protein S3 (RPS3))

Protein Type: Recombinant

Protein Characteristics: AA 2-243

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This RPS3 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequence: AVQISKKRKF VADGIFKAEL NEFLTRELAE DGYSGVEVRV TPTRTEIIIL ATRTQNVLGE KGRRIRELTA VVQKRFGFPE GSVELYAEKV ATRGLCAIAQ AESLRYKLLG GLAVRRACYG VLRFIMESGA KGCEVVVSGK LRGQRAKSMK FVDGLMIHSG DPVNYYVDTA VRHVLLRQGV LGIKVKIMLP WDPSGKIGPK KPLPDHVSIV EPKDEILPTT PISEQKGGKP EPPAMPQPVP TA
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Rps3 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: RPS3 (Ribosomal Protein S3 (RPS3))

Alternative Name: Rps3 (RPS3 Products)

Synonyms: S3 Protein, D7Ertd795e Protein, Rs_3 Protein, fb13d09 Protein, wu:fb13d09 Protein, zgc:56088 Protein, ribosomal protein S1a Protein, rps3 Protein, 6779 Protein, CG6779 Protein, DmRpS3 Protein, Dmel\\CG6779 Protein, M(3)124 Protein, M(3)95A Protein, M(3)B Protein, M(3)B2 Protein, M(3)Fla Protein, M(3)w Protein, M(3R)w Protein, M95A Protein, M[[w]] Protein, Mw Protein, RPS3 Protein, RS3 Protein, Rp S3 Protein, RpS Protein, Rps3 Protein, X72921 Protein, dS3 Protein, rpS3 Protein, rpls1b Protein, rpls3-b Protein, ribosomal protein S3 Protein, 40S ribosomal protein S3 Protein, ribosomal protein S3 L homeolog Protein, Ribosomal protein S3 Protein, 30S ribosomal protein S3 Protein, ribosomal protein S3 S homeolog Protein, RPS3 Protein, Rps3 Protein, rps-3 Protein, rps3 Protein, rps3.L Protein, RpS3 Protein, rps3.S Protein

Background: Involved in translation as a component of the 40S small ribosomal subunit (By similarity). Has endonuclease activity and plays a role in repair of damaged DNA (PubMed:7775413). Cleaves phosphodiester bonds of DNAs containing altered bases with broad specificity and cleaves supercoiled DNA more efficiently than relaxed DNA (By similarity). Displays high binding affinity for 7,8-dihydro-8-oxoguanine (8-oxoG), a common DNA lesion caused by reactive oxygen species (ROS) (By similarity). Has also been shown to bind with similar affinity to intact and damaged DNA (By similarity). Stimulates the N-glycosylase activity of the base excision protein OGG1 (By similarity). Enhances the uracil excision activity of UNG1 (By similarity). Also stimulates the cleavage of the phosphodiester backbone by APEX1 (By similarity). When located in the mitochondrion, reduces cellular ROS levels and mitochondrial DNA damage (By similarity). Has also been shown to negatively regulate DNA repair in cells exposed to hydrogen peroxide (By similarity). Plays a role in regulating transcription as part of the NF-kappa-B p65-p50 complex where it binds to the RELA/p65 subunit, enhances binding of the complex to DNA and promotes transcription of target genes (By similarity). Represses its own translation by binding to its cognate mRNA (By similarity). Binds to and protects TP53/p53 from MDM2-mediated ubiquitination (By similarity). Involved in spindle formation and chromosome movement during mitosis by regulating microtubule polymerization (By similarity). Involved in induction of apoptosis through its role in activation of CASP8 (PubMed:14988002). Induces neuronal apoptosis by interacting with the E2F1 transcription factor and acting synergistically with it to up-regulate pro-apoptotic proteins BCL2L11/BIM and HRK/Dp5 (By similarity). Interacts with TRADD following exposure to UV radiation and induces apoptosis by caspase-dependent JNK activation (By similarity). {ECO:0000250|UniProtKB:P23396, ECO:0000269|PubMed:14988002, ECO:0000269|PubMed:7775413}.

Molecular Weight: 27.5 kDa Including tag.

UniProt: P62908

Pathways: Positive Regulation of Endopeptidase Activity