PKC beta Protein (AA 2-671) (His tag)

Catalog No. ABIN3134607

Product Details

Target: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Protein Type: Recombinant

Protein Characteristics: AA 2-671

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This PKC beta protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: ADPAAGPPPS EGEESTVRFA RKGALRQKNV HEVKNHKFTA RFFKQPTFCS HCTDFIWGFG KQGFQCQVCC FVVHKRCHEF VTFSCPGADK GPASDDPRSK HKFKIHTYSS PTFCDHCGSL LYGLIHQGMK CDTCMMNVHK RCVMNVPSLC GTDHTERRGR IYIQAHIDRE VLIVVVRDAK NLVPMDPNGL SDPYVKLKLI PDPKSESKQK TKTIKCSLNP EWNETFRFQL KESDKDRRLS VEIWDWDLTS RNDFMGSLSF GISELQKAGV DGWFKLLSQE EGEYFNVPVP PEGSEGNEEL RQKFERAKIG QGTKAPEEKT ANTISKFDNN GNRDRMKLTD FNFLMVLGKG SFGKVMLSER KGTDELYAVK ILKKDVVIQD DDVECTMVEK RVLALPGKPP FLTQLHSCFQ TMDRLYFVME YVNGGDLMYH IQQVGRFKEP HAVFYAAEIA IGLFFLQSKG IIYRDLKLDN VMLDSEGHIK IADFGMCKEN IWDGVTTKTF CGTPDYIAPE IIAYQPYGKS VDWWAFGVLL YEMLAGQAPF EGEDEDELFQ SIMEHNVAYP KSMSKEAVAI CKGLMTKHPG KRLGCGPEGE RDIKEHAFFR YIDWEKLERK EIQPPYKPKA RDKRDTSNFD KEFTRQPVEL TPTDKLFIMN LDQNEFAGFS YTNPEFVINV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Prkcb Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Alternative Name: Prkcb (PRKCB Products)

Synonyms: PKC-beta Protein, PKCB Protein, PRKCB1 Protein, PRKCB2 Protein, A130082F03Rik Protein, PKC-Beta Protein, Pkcb Protein, Prkcb1 Protein, Prkcb2 Protein, PKC-B Protein, Prkcb Protein, prkcb1 Protein, zgc:63591 Protein, PKC Protein, PRKCB_tv2 Protein, Pkc53E Protein, prkcb1l Protein, zgc:64063 Protein, protein kinase C beta Protein, protein kinase C, beta Protein, protein kinase C, beta b Protein, protein kinase C, beta a Protein, PRKCB Protein, Prkcb Protein, prkcbb Protein, prkcba Protein

Background: Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. May participate in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis. Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription (By similarity). {ECO:0000250, ECO:0000269|PubMed:10499500, ECO:0000269|PubMed:11805327, ECO:0000269|PubMed:12070292, ECO:0000269|PubMed:12118249, ECO:0000269|PubMed:16301747, ECO:0000269|PubMed:16356855, ECO:0000269|PubMed:16505232, ECO:0000269|PubMed:17272725, ECO:0000269|PubMed:8670417}.

Molecular Weight: 77.6 kDa Including tag.

UniProt: P68404

Pathways: WNT Signaling, TCR Signaling, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded Protein Response, BCR Signaling