BACH2 Protein (AA 1-839) (Strep Tag)

Catalog No. ABIN3134679

Product Details

Target: BACH2 (BTB and CNC Homology 1, Basic Leucine Zipper Transcription Factor 2 (BACH2))

Protein Type: Recombinant

Protein Characteristics: AA 1-839

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This BACH2 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MSVDEKPGSP MYVYESTVHC ANILLGLNDQ RKKDILCDVT LIVERKEFRA HRAVLAACSE YFWQALVGQT KDDLVVSLPE EVTARGFGPL LQFAYTAKLL LSRENIREVI RCAEFLRMHN LEDSCFSFLQ TQLLNREDGL FVCRKDSACQ RPQEDHGNSA GEEEEEEETM DSETARMACA TDQMLPDPIS FEATAIPVAE KEEALLPESE VPTDTKENSE KGALTQYPRY KKYQLACTKN VYSAPSHGTS GFASTFSEDS PGNSLKPGLP MGQIKSEPPS EETEEESITL CLSGDETDIK DRPGDVEMDR KQPSPAPTPS TPTGAACLDR SRSVSSPSCL RSLFGITKGV ESTGLPSTSQ QPLVRSSACP FNKGISQGDL KTDYTPLAGN YGQPHVGQKD VSNFAMGSPL RGPGPETLCK QEGELDRRSV IFSASACDQP NTPVHSYSAV SNLDKDLSEP VPKSLWVGAG QSLPSSQAYS HSGLMADHLP GRIRPNTSCP VPIKVCPRSP PLETRTRTSS SCSSYSYAED GSGGSPCSLP LCEFSSSPCS QGARFLATEH QEPGLMGDGM YNQVRPQIKC EQSYGTNSSD ESGSFSEADS ESCPVQDRGQ EVKLPFPVDQ ITDLPRNDFQ MMIKMHKLTS EQLEFIHDIR RRSKNRIAAQ RCRKRKLDCI QNLECEIRKL VCEKEKLLSE RNHLKACMGE LLDNFSCLSQ EVCRDIQSPE QIQALHRYCP VLIPMDLPGA SVNPPPVGVE QSLAPSPCAV GGSVPCCLEP GAAPPGLPWV PSNTSENCTS GRRLEGSDPG TFSERGPPLE ARSQSVTVDF CQEMTEKCTT DEQPRKDYA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: BACH2 (BTB and CNC Homology 1, Basic Leucine Zipper Transcription Factor 2 (BACH2))

Alternative Name: Bach2 (BACH2 Products)

Synonyms: E030004N02Rik Protein, Bach2 Protein, BTBD25 Protein, bach2 Protein, si:dkeyp-4f12.1 Protein, si:dkeyp-4f12.2 Protein, BTB and CNC homology, basic leucine zipper transcription factor 2 Protein, BTB domain and CNC homolog 2 Protein, BTB and CNC homology 1, basic leucine zipper transcription factor 2b Protein, Bach2 Protein, BACH2 Protein, bach2b Protein, bach2 Protein

Background: Transcription regulator protein BACH2 (BTB and CNC homolog 2),FUNCTION: Transcriptional regulator that acts as a repressor or activator (PubMed:8887638). Binds to Maf recognition elements (MARE) (PubMed:8887638). Plays an important role in coordinating transcription activation and repression by MAFK (PubMed:8887638). Induces apoptosis in response to oxidative stress through repression of the antiapoptotic factor HMOX1 (By similarity). Positively regulates the nuclear import of actin (PubMed:26021350). Is a key regulator of adaptive immunity, crucial for the maintenance of regulatory T-cell function and B-cell maturation (PubMed:28530713). {ECO:0000250|UniProtKB:Q9BYV9, ECO:0000269|PubMed:26021350, ECO:0000269|PubMed:28530713}.

Molecular Weight: 91.8 kDa

UniProt: P97303