NFATC3 Protein (AA 2-1075) (His tag)

Catalog No. ABIN3134680

Product Details

Target: NFATC3 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 3 (NFATC3))

Protein Type: Recombinant

Protein Characteristics: AA 2-1075

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This NFATC3 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: TTANCGAHDE LDFKLVFGED GAPAPPPPGS RPADLEPDDC ASIYIFNVDP PPSTLTTPLC LPHHGLPSHS SVLSPSFQLQ SHKNYEGTCE IPESKYSPLG GPKPFECPSI QFTSISPNCQ QELDAHEDDL QINDPEREFL ERPSRDHLYL PLEPSYRESS LSPSPASSIS SRSWFSDASS CESLSHIYDD VDSELNEAAA RFTLGSPLTS PGGSPGGCPG EESWHQQYGS GHSLSPRQSP CHSPRSSITD ENWLSPRPAS GPSSRPTSPC GKRRHSSAEV CYAGSLSPHH SPVPSPGHSP RGSVTEDTWL TAPVHTGSGL SPAPFPFQYC VETDIPLKTR KTSEDQAAIL PGKLEICSDD QGNLSPSRET SVDDGLGSQY PLKKDSSGDQ FLSVPSPFTW SKPKPGHTPI FRTSSLPPLD WPLPTHFGQC ELKIEVQPKT HHRAHYETEG SRGAVKASTG GHPVVKLLGY SEKPINLQMF IGTADDRYLR PHAFYQVHRI TGKTVATASQ EIIIASTKVL EIPLLPENNM SASIDCAGIL KLRNSDIELR KGETDIGRKN TRVRLVFRVH IPQPSGKVLS LQIASIPVEC SQRSAQELPH IEKYSINSCS VNGGHEMIVT GSNFLPESKI IFLEKGQDGG PHWEVEGKII REKCQGAHIV LEVPPYHNPA VTSAVQVHFY LCNGKRKKSQ SQRFTYTPVL MKQEQREDTD LPSVPSLPVP HSAQAQRPSS ETGHPHDRAM SAPGGLLCQV QPAYTSMVAS THLPQLQCRD EGAGKEQHIA TSSVMHQPFQ VTPTSPIGSS YQSIQTSMYN GPTCLPVNPA SSQEFDPVLF QQDAALSSLV NLGCQPLSPI PFHSSNSDAT GHLLAHSPHS VQTPPHLQSM GYHCSNAGQT ALSSPVADQI TGQPSSHLQP ITYCPSHPGS ATAASPAASH PLASSPISGP SSPQLQPMPY QSPSSGTASS PSPTTRMHSG QHSTQAQSTG QGGLSVPSSL VCHSLCDPAS FPPGGATVSI KPEPEDQEPN FATIGLQDIT LDDVNEIIGR DMSQISVSQA TEVMRDTPLP GPASPDLMTS HSAH
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Nfatc3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: NFATC3 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 3 (NFATC3))

Alternative Name: Nfatc3 (NFATC3 Products)

Synonyms: NFATC3 Protein, NFATx Protein, nf-at Protein, nfat4 Protein, nfatx Protein, C80703 Protein, D8Ertd281e Protein, NFAT4 Protein, NFATX Protein, nuclear factor of activated T-cells 3 Protein, nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3 Protein, nuclear factor of activated T-cells 3 S homeolog Protein, NFATC3 Protein, nfatc3 Protein, Nfatc3 Protein, nfatc3.S Protein

Background: Acts as a regulator of transcriptional activation. Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 (By similarity). {ECO:0000250}.

Molecular Weight: 116.3 kDa Including tag.

UniProt: P97305

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Chromatin Binding