NBR1 Protein (AA 1-988) (Strep Tag)

Catalog No. ABIN3134699

This Recombinant NBR1 protein is produced in Cell-free protein synthesis (CFPS).

Quick Overview for NBR1 Protein (AA 1-988) (Strep Tag) (ABIN3134699)

Target: NBR1 (Neighbor of BRCA1 Gene 1 (NBR1))

Protein Type: Recombinant

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-988

Purification tag / Conjugate: This NBR1 protein is labelled with Strep Tag.

Sequence: MEPQVTLNVT FKNETQSFLV SDPENTTWAD VEAMVKVSFD LNTIQIKYLD EENEEISINS QGEYEEALKM ANIKQGNQLQ MQVHEGYHVV DEALPKNVVE NQAAARTGKK PLAHYSSLVR VLGSDMKTTE EPAPEQCSSA PCDTDQPQDK PPDWFTSYLE MFREQVVKET VEKLEQRLQE KLVLQKPLLS SSPTEVSMPI SEETLFLPEN QFSWHIACSH CQKRIVGVRY QCSLCPSYNI CEDCEAGPYT HDTNHVLLKL RRPVVISSEP FFYSKYSAPR LPAALEQVRL QKQVDKNFVK AEKQRLRAEK KQRKAEVKEL KKQLKLHRKI HLWNSIHGLQ SPKSPLGRPE SLLQSNTLML PLQPCAPVMP TLSAAFVDEN LPDGTHLQPG TKFIKHWRMK NTGNVKWNTD TKLKFMWGNL TLASTEKKDV LVPCLKAGHV GVVSVEFIAP TLEGTYTSHW RLSHKGQQFG PRVWCSIIVD PFPSSESPDN VEGDRISSSK ADDFSCEQEE AFLLAEEEIP LGEVTKQTEG TGASASQKTR RAASERELYI PSVDLLTAQD LLSFELLDIN IVQELERVPH NTPVDMTPCM SPLPHDSPLI EKPGLGQIQE ESEGAGFKAP PDSTVSAKRK AETPASVEET EEDLSGTQFV CETVIRSLTL DAAPDHNPPC RQRSPQRELQ LYSTEGQQPL VLPGFCRKDS SLKFALPEEG PRGDEREEIV HIVEEEVVEE EEEVQDEEVQ SQSSASSEDY IIILPECFDT SRPLGDSMYS SALSQPGLER GAEGEPGIES GLEPTEARER LPERESQPQE QSISDILTTS QPLDTVPLVP EVAGLPAALS RSAPCGQCES SGVDSPGVDS PATMHEVPPA PDDIRGEPRG STGLANSRQR SCDHSRHHNG SSIAGGLVKG ALSVAASAYK ALFSGPPVTA QPIVSEDQTT ALMAHLFEMG FCDRQLNLRL LRKHNYNILQ VVTELLQVNN NDWYSHRY
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: NBR1 (Neighbor of BRCA1 Gene 1 (NBR1))

Alternative Name: Nbr1

Background: Next to BRCA1 gene 1 protein (Membrane component chromosome 17 surface marker 2 homolog) (Neighbor of BRCA1 gene 1 protein),FUNCTION: Ubiquitin-binding autophagy adapter that participates in different processes including host defense or intracellular homeostasis (PubMed:31916398, PubMed:34374750). Possesses a double function during the selective autophagy by acting as a shuttle bringing ubiquitinated proteins to autophagosomes and also by participating in the formation of protein aggregates. Plays a role in the regulation of the innate immune response by modulating type I interferon production and targeting ubiquitinated IRF3 for autophagic degradation (By similarity). In response to oxidative stress, promotes an increase in SQSTM1 levels, phosphorylation, and body formation by preventing its autophagic degradation (PubMed:31916398). In turn, activates the KEAP1-NRF2/NFE2L2 antioxidant pathway (PubMed:31916398). Plays also non-autophagy role by mediating the shuttle of IL-12 to late endosome for subsequent secretion (PubMed:34374750). {ECO:0000250|UniProtKB:Q14596, ECO:0000269|PubMed:31916398, ECO:0000269|PubMed:34374750}.

Molecular Weight: 110.0 kDa

UniProt: P97432

Pathways: Autophagy