CD13 Protein (AA 33-966) (His tag)

Catalog No. ABIN3134707

Product Details

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Protein Type: Recombinant

Protein Characteristics: AA 33-966

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This CD13 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequence: YAQEKNRNAE NSATAPTLPG STSATTATTT PAVDESKPWN QYRLPKTLIP DSYRVILRPY LTPNNQGLYI FQGNSTVRFT CNQTTDVIII HSKKLNYTLK GNHRVVLRTL DGTPAPNIDK TELVERTEYL VVHLQGSLVE GRQYEMDSQF QGELADDLAG FYRSEYMEGD VKKVVATTQM QAADARKSFP CFDEPAMKAM FNITLIYPNN LIALSNMLPK ESKPYPEDPS CTMTEFHSTP KMSTYLLAYI VSEFKNISSV SANGVQIGIW ARPSAIDEGQ GDYALNVTGP ILNFFAQHYN TSYPLPKSDQ IALPDFNAGA MENWGLVTYR ESSLVFDSQS SSISNKERVV TVIAHELAHQ WFGNLVTVAW WNDLWLNEGF ASYVEYLGAD YAEPTWNLKD LMVLNDVYRV MAVDALASSH PLSSPADEIK TPDQIMELFD SITYSKGASV IRMLSSFLTE DLFKKGLSSY LHTYQYSNTV YLDLWEHLQK AVNQQTAVQP PATVRTIMDR WILQMGFPVI TVNTNTGEIS QKHFLLDSKS NVTRPSEFNY IWIAPIPFLK SGQEDHYWLD VEKNQSAKFQ TSSNEWILLN INVTGYYLVN YDENNWKKLQ NQLQTDLSVI PVINRAQIIH DSFNLASAKM IPITLALDNT LFLVKEAEYM PWQAALSSLN YFTLMFDRSE VYGPMKRYLK KQVTPLFFYF QNRTNNWVNR PPTLMEQYNE INAISTACSS GLKECRDLVV ELYSQWMKNP NNNTIHPNLR STVYCNAIAF GGEEEWNFAW EQFRNATLVN EADKLRSALA CSKDVWILNR YLSYTLNPDY IRKQDTTSTI ISIASNVAGH PLVWDFVRSN WKKLFENYGG GSFSFANLIQ GVTRRFSSEF ELQQLEQFKA DNSATGFGTG TRALEQALEK TRANIDWVKE NKDAVFKWFT ENSS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Anpep Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Alternative Name: Anpep (ANPEP Products)

Synonyms: APN Protein, CD13 Protein, GP150 Protein, LAP1 Protein, P150 Protein, PEPN Protein, A2AP Protein, AAP Protein, ALPHA-2-PI Protein, API Protein, PLI Protein, AAT1 Protein, ALT1 Protein, GPT1 Protein, Gpt1 Protein, Apn Protein, pepN Protein, SC8E4A.13 Protein, 1300007J06Rik Protein, 2310022B03Rik Protein, ALT Protein, Gpt-1 Protein, AP-M Protein, AP-N Protein, Cd13 Protein, Apm Protein, KZP Protein, Lap1 Protein, rAPN Protein, APDE Protein, p150 Protein, anpep Protein, fb64c05 Protein, wu:fb64c05 Protein, zgc:136771 Protein, alanyl aminopeptidase, membrane Protein, serpin family F member 2 Protein, glutamic--pyruvic transaminase Protein, aminopeptidase N Protein, aminopeptidase Protein, aminopeptidase n Protein, glutamic pyruvic transaminase, soluble Protein, alanyl (membrane) aminopeptidase Protein, membrane alanyl aminopeptidase Protein, alanyl (membrane) aminopeptidase b Protein, alanyl aminopeptidase, membrane L homeolog Protein, ANPEP Protein, SERPINF2 Protein, GPT Protein, Gpt Protein, Apn2 Protein, Apn1 Protein, SCO2643 Protein, pepN Protein, Shewana3_0897 Protein, Alvin_1024 Protein, Ndas_3413 Protein, Saut_1544 Protein, Fbal_1877 Protein, ML5_3424 Protein, Despr_1988 Protein, Mesop_5365 Protein, Anpep Protein, LOC101744250 Protein, anpepb Protein, anpep.L Protein

Background: Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May have a role in angiogenesis (By similarity). Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells. {ECO:0000250, ECO:0000269|PubMed:8103749}.

Molecular Weight: 107.4 kDa Including tag.

UniProt: P97449

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones