ARVCF Protein (AA 1-962) (His tag)
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- Target See all ARVCF Proteins
- ARVCF (Armadillo Repeat Gene Deleted in Velocardiofacial Syndrome (ARVCF))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-962
- Origin
- Mouse
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Source
- Insect Cells
- Purification tag / Conjugate
- This ARVCF protein is labelled with His tag.
- Application
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Sequence
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MEDCNVHSAA SILASVKEQE ARFERLTRAL EQERRHVALQ LERAQQPGMS SGGMVGSGQP LPMAWQQLVL QEQSPGSQAS LATMPEAPEV LEETVTVEED PGTPTSHVSI VTSEDGTTRR TETKVTKTVK TVTTRTVRQV PLGPDGLPLL DGGPPLGSFA DGPLDRHYLL RGGGGPAATL SRTYHSSGGG FPDGPESRDI PSYGSLSRGL GVRPPRTGLL GPGPGDGCFT LPGRREAFPM GSESGPPSGR SLPEHFQAEP YGLEDDTRSL AADDEGGPDL EPDYSTATRR RPEYGRGLRA RAFEDTADDA GELIEERPPF PAATAPLAQP ERGSLGSLDR VVRRSPSVDS TRKEPRWRDP ELPEVLAMLR HPVDPVKANA AAYLQHLCFE NEGIKRRVRQ LRGLPLLVAL LDHPRAEVRR RACGALRNLS YGRDTDNKAA IRDCGGVPAL VRLLRAARDN EVRELVTGTL WNLSSYEPLK MVIIDHGLQT LTHEVIVPHS GWEREPNEDS KPRDAEWTTV FKNTSGCLRN VSSDGAEARR RLRECEGLVD ALLHALQSAV GRKDTDNKSV ENCVCIMRNL SYHVHKEVPG ADRYQEAEPG IPGSTTSQRR RKDDASCFGG KKAKEEWFHQ GKKDAEMDRN FDTLDLPKRT EAAKGFELLY QPEVVRLYLS LLTESRNFNT LEAAAGALQN LSAGNWTWAT YIRATVRKER GLPVLVELLQ SETDKVVRAV AIALRNLSLD QRNKDLIGSY AMTELVRNVR NAQAPAHPSA HLEEDTVVAV LNTIHEIVSD SLDNARSLLQ ARGVPALVAL VASSQSVREA KAASHVLQTV WSYKELRGAL QRDGWTKSRF QSASTAKGPK GTPSSGGFDD STLPLVDKSL DGEKSNTRDV IPMDTLGPDG YATVDRRERR TLGSDSTGDT SEKELLRPDP GRKAPPPGPS RPSVRLVDAV GDTKPQPVDS WV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Arvcf Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product ARVCF Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- ARVCF (Armadillo Repeat Gene Deleted in Velocardiofacial Syndrome (ARVCF))
- Alternative Name
- Arvcf (ARVCF Products)
- Synonyms
- arvcf Protein, p120 Protein, MGC79880 Protein, ARVCF Protein, Xarvcf Protein, armadillo repeat gene deleted in velocardiofacial syndrome Protein, ARVCF, delta catenin family member L homeolog Protein, ARVCF, delta catenin family member Protein, ARVCF Protein, arvcf.L Protein, arvcf Protein, Arvcf Protein
- Background
- Involved in protein-protein interactions at adherens junctions. {ECO:0000250}.
- Molecular Weight
- 106.0 kDa Including tag.
- UniProt
- P98203
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