COL1A2 Protein (AA 86-1108) (His tag)

Catalog No. ABIN3134764

Product Details

Target: COL1A2 (Collagen, Type I, alpha 2 (COL1A2))

Protein Type: Recombinant

Protein Characteristics: AA 86-1108

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This COL1A2 protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: QYSDKGVSSG PGPMGLMGPR GPPGAVGAPG PQGFQGPAGE PGEPGQTGPA GPRGPAGSPG KAGEDGHPGK PGRPGERGVV GPQGARGFPG TPGLPGFKGV KGHSGMDGLK GQPGAQGVKG EPGAPGENGT PGQAGARGLP GERGRVGAPG PAGARGSDGS VGPVGPAGPI GSAGPPGFPG APGPKGELGP VGNPGPAGPA GPRGEVGLPG LSGPVGPPGN PGTNGLTGAK GATGLPGVAG APGLPGPRGI PGPAGAAGAT GARGLVGEPG PAGSKGESGN KGEPGSVGAQ GPPGPSGEEG KRGSPGEAGS AGPAGPPGLR GSPGSRGLPG ADGRAGVMGP PGNRGSTGPA GIRGPNGDAG RPGEPGLMGP RGLPGSPGNV GPSGKEGPVG LPGIDGRPGP IGPAGPRGEA GNIGFPGPKG PSGDPGKPGE RGHPGLAGAR GAPGPDGNNG AQGPPGPQGV QGGKGEQGPA GPPGFQGLPG PSGTTGEVGK PGERGLPGEF GLPGPAGPRG ERGTPGESGA AGPSGPIGSR GPSGAPGPDG NKGEAGAVGA PGSAGASGPG GLPGERGAAG IPGGKGEKGE TGLRGDTGNT GRDGARGIPG AVGAPGPAGA SGDRGEAGAA GPSGPAGPRG SPGERGEVGP AGPNGFAGPA GAAGQPGAKG EKGTKGPKGE NGIVGPTGSV GAAGPSGPNG PPGPVGSRGD GGPPGMTGFP GAAGRTGPPG PSGIAGPPGP PGAAGKEGIR GPRGDQGPVG RTGETGASGP PGFVGEKGPS GEPGTAGAPG TAGPQGLLGA PGILGLPGSR GERGLPGIAG ALGEPGPLGI SGPPGARGPP GAVGSPGVNG APGEAGRDGN PGSDGPPGRD GQPGHKGERG YPGSIGPTGA AGAPGPHGSV GPAGKHGNRG EPGPAGSVGP VGAVGPRGPS GPQGIRGDKG EPGDKGHRGL PGLKGYSGLQ GLPGLAGLHG DQGAPGPVGP AGPRGPAGPS GPVGKDGRSG QPGPVGPAGV RGSQGSQGPA GPPGPPGPPG PPG
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Col1a2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: COL1A2 (Collagen, Type I, alpha 2 (COL1A2))

Alternative Name: Col1a2 (COL1A2 Products)

Synonyms: AA960264 Protein, AI325291 Protein, Col1a-2 Protein, Cola-2 Protein, Cola2 Protein, oim Protein, OI4 Protein, Gas-6 Protein, alpha2(I) Protein, hm:zehn2357 Protein, wu:fa98d05 Protein, wu:fa99g10 Protein, wu:fb04c08 Protein, wu:fb11d06 Protein, zehn2357 Protein, coagulation factor II, thrombin Protein, collagen, type I, alpha 2 Protein, collagen type I alpha 2 chain Protein, collagen type I alpha 2 L homeolog Protein, growth arrest specific 6 Protein, F2 Protein, Col1a2 Protein, col1a2.L Protein, COL1A2 Protein, Gas6 Protein, col1a2 Protein

Background: Type I collagen is a member of group I collagen (fibrillar forming collagen).

Molecular Weight: 92.7 kDa Including tag.

UniProt: Q01149