PREX2 Protein (AA 1-1598) (Strep Tag)

Catalog No. ABIN3134959

Product Details

Target: PREX2 (DEPDC2) (DEP Domain Containing 2 (DEPDC2))

Protein Type: Recombinant

Protein Characteristics: AA 1-1598

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This PREX2 protein is labelled with Strep Tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS)

Sequence: MSDESAREVD KQLRLRVCVL SELQKTERDY VGTLEFLVSA FLHRMNQCAA AKVDKNVTEE TVKMLFSNIE EILIVHKEFL KVVEECLYPE PSAQQEVGAC FLHFKDKFRI YDEYCSNHEK AQKLLLELNK IRTIRTFLLN CMLLGGRKNT DVPLEGYLVT PIQRICKYPL LLKELLKRTP RRHSDYTAVM EALQAMKAVC SNINEAKRQM EKLEVLEEWQ AHIEGWEGSN ITDTCTEMLM CGVLMKISSG NIQERVFFLF DNLLVYCKRK HRRLKNSKAS TDGYRYVFRG RINTEVMEVE NVDDGTADFH SSGHIVVNGW KIHNTAKNKW FVCMAKSPEE KHEWFEAILK ERERRKGLKL GMEQDTWVMI SEQGEKLYKM MCKQGNLIKD RKRKLTTFPK CFLGSEFVSW LLEIGEIHRP EEGVHLGQAL LENGIIHHVT DKHQFKPEQM LYRFRYDDGT FYPRSEMQDV ISKGVRLYCR LHSLFTPVVR DKDYHLRTYK SVVMANKLID WLIAQGDCRT REEAMIFAVG LCDNGFMHHV LEKSEFKDEP LLFRFFADEE MEGSNMKHRL MKHDLKVVEN VIAKSLLIKS NEGSYGFGLE DKNKVPIIKL VEKGSNAEMA GMEVGKKIFA INGDLVFLRP FPEVDCFLKS CLNSRKPLRV LVSTKPRETV KIPDSADGLG FQIRGFGPSV VHAVGRGTVA AAAGLHPGQC IIKVNGINVS KETHASVIAH VTACRKYKRP MKQDSIQWVY DSLESAQEDI QKSHSKPPGD GAGDAFECKV EDVIDKFNTM AIIDGKKEHV SLTVDNVHLE YGVVYEYDST AGTKCNVVEK MVEPKGFFSL TAKILEALAK SDEHFVQNCT SLNSLNEVIA TDLQSKFTSM CSERIEHVCH RISSYGRFSR VLKNRAWPTF KQAKPKISPL HSSDFCPTNC HVNVMEVSYP KTSTSLGSAF GVQLDSRKHN SHDKENKSVE PGKLSPMVYI QHTITTMAAP SGLSLGHKDG HGLQYLLKEE DLETQDIYHK LLGKLQTALK EVEMSVCQID DLLSSITYSP KLERKTTECV TPMDSDNEKG ERNSKRVCFN VAGDEQEDSG HDTVSNRDSY SDCNSNRNSI ASFTSICSSQ CSSYFHSDEM DSGDELPISV RISHDKQDKI HTCLEQLFSQ IDSIINLLKG QAVIRAFEQT KYLTPGRGLQ EFQQEMEAKL SCPRRLRLHL KQDPWNLPSS IQALAQSIRK HAEEVKCRIL LALLEYSDSE TQLRRDMVFC QSLVATVCAF SEQLMAALNQ MFDNSKENEM ETCEASRRWL DQIANAGVLF HFQSLLSPNL KDEQAMLEDT LVALFDLEKV SFFFKPSEED PLVANVPLTY QVEGSRQALK VYFYMDSYHF EQLPQRLKNG GGFKIHPVLF SQALESMEGY CYRDNISVEE FQAQINTASL EKVKQYNQKL RAFYLDKSNS PPNTTSKAAY IDKLMKPLNA LDELYRLITS FIRSKRIAAC VNTPCSASGV GLLSVSSELC DRLGACHIIM CSSGVHRCTL SVTLEQTITL ARSHGLPPRY IMQAMDVMRK QGARVQNTAK NLGVRDRTPQ SAPRLYKLCE PPPPVGEE
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PREX2 (DEPDC2) (DEP Domain Containing 2 (DEPDC2))

Alternative Name: Prex2 (DEPDC2 Products)

Synonyms: 6230420N16Rik Protein, DEP.2 Protein, DEPDC2 Protein, P-REX2 Protein, AI316880 Protein, AI553603 Protein, C030045D06Rik Protein, D430013K02 Protein, Depdc2 Protein, P-Rex2 Protein, phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 2 Protein, phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 Protein, PREX2 Protein, Prex2 Protein

Background: Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein (P-Rex2) (PtdIns(3,4,5)-dependent Rac exchanger 2) (DEP domain-containing protein 2),FUNCTION: Functions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. Mediates the activation of RAC1 in a PI3K-dependent manner. May be an important mediator of Rac signaling, acting directly downstream of both G protein-coupled receptors and phosphoinositide 3-kinase (By similarity). {ECO:0000250}.

Molecular Weight: 181.7 kDa

UniProt: Q3LAC4