GSAP Protein (AA 1-858) (His tag)
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- Target See all GSAP (PION) Proteins
- GSAP (PION) (Pigeon Homolog (PION))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-858
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Origin
- Mouse
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Source
- Insect Cells
- Purification tag / Conjugate
- This GSAP protein is labelled with His tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- Sequence
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MALRLVTHFD VLEDVLPSLL TQAATTDEGD RAGVLETTYG SLRVLNIERN GNIIYTYKDN KGNAVFGLYD CQTRQNEHLY TFEKDMQAVS CSVNSERTVL AASFIQYTTE GVKNDLQPGS KCLTLLVEIH PVNNVKVLKA VDSCVWVQFL YPQAESHLLP QNHLLLISEE KYIERFHIQI TREDGDRVVI RNSSHLPRDR LAEDFVWAQW DLSEQRLYYI ELKESRSILK CIQFRADESF NLMFEMPLDI TLTGLRFKLV NFGYDYRQDR EKLCNQPSLC IFTNHTGSLC MCYSPKSDSR EEITYSVFYL HKGYRKIFTA APGSADSQVT NGADSQVTDG IAFLNLGYFV AVYSPGHFLH LLNIQHPDLV CHSLFLTGNN KIAAVLPPSP LQSLPGSLVL DCYSGKVYRV TLDQSYLLRF LWNAHLDCER MAALHCILSC SQDPGFPEEQ IIQWISEHVS ACHSFDLIQE FLIASSYWSV YAELDDMGML LQYSSVLTWN TEIPGIKFTT EELPLPLMKV YGLKGYWAKL NSNLEYIKYT KPHLHYHNSV VRREWHNLIS EERTGKRRST MYVRNILENA MKVIASMETR TLEPRLIPFL QEEDRHQRLL MGLMVSELRD HLLRHLQGVE KKKIEQMVLD YISKLLDLIW CLLETSWRKH SMHPLVLHLN SHCSAADFEV FHLMTRILDA ASSLCLPLPP GFHSLHTILG VHCLPLYSLL HYIDNGVLLL TETAVTRLMK DLDNSEKNEQ LKFSIIVRLP PLIGQKVCRL WDHPMSSNII SRNHVARLLK NYRKEPRNSM IDKSSFPVEF LPLNYFIEIL MGLESSNQAL YGFEGHDNVD AEFVEEAALK HTTMLLGL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Gsap Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product PION Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- GSAP (PION) (Pigeon Homolog (PION))
- Alternative Name
- Gsap (PION Products)
- Synonyms
- PION Protein, A530088I07Rik Protein, Pion Protein, gamma-secretase activating protein Protein, GSAP Protein, Gsap Protein
- Background
- Regulator of gamma-secretase activity, which specifically activates the production of beta-amyloid protein (beta-amyloid protein 40 and beta-amyloid protein 42), without affecting the cleavage of other gamma-secretase targets such has Notch. The gamma-secretase complex is an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Specifically promotes the gamma-cleavage of APP CTF-alpha (also named APP-CTF) by the gamma-secretase complex to generate beta-amyloid, while it reduces the epsilon-cleavage of APP CTF-alpha, leading to a low production of AICD. {ECO:0000269|PubMed:20811458}.
- Molecular Weight
- 99.6 kDa Including tag.
- UniProt
- Q3TCV3
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