RASIP1 Protein (AA 1-961) (Strep Tag)

Catalog No. ABIN3135004

Product Details

Target: RASIP1 (Ras Interacting Protein 1 (RASIP1))

Protein Type: Recombinant

Protein Characteristics: AA 1-961

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This RASIP1 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MLSGERKEGG SPRFGKLHLP VGLWINSPRK QLAKLGRRWP SAASVKSSSS DTGSRSSEPL PPPPPPPHVE LRRVGAVKAA GGASGSRAKR ISQLFRGSGA GGAGGPGTPG GAQRWASEKK LPELAAGVAP EPPLPTRAAV PPGVLKIFAS GLASGANYKS VLATERSTAR ELVAEALERY GLTGGRGAGD SGCVDAYALC DALGRPAVGV GGGEWRAEHL RVLADAERPL LVQDLWRARP GWARRFELRG REEARRLEQE AFGAADADGT NAPSWRTQKN RSRAASGGAA LASPGPGSGS GTPTGSGGKE RSENLSLRRS VSELSLQGRR RRQQERRQQA LSMAPGAADA QMVPTDPGDF DQLTQCLIQA PSNRPYFLLL QGYQDAQDFV VYVMTREQHV FGRGGPSSSR GGSPAPYVDT FLNAPDILPR HCTVRAGPEP PAMVRPSRGA PVTHNGCLLL REAELHPGDL LGLGEHFLFM YKDPRSGGSG PARPSWLPAR PGAAPPGPGW AFSCRLCGRG LQERGEALAA YLDGREPVLR FRPREEEALL GEIVRAAASG AGDLPPLGPA TLLALCVQHS ARELELGHLP RLLGRLARLI KEAVWEKIKE IGDRQPENHP EGVPEVPLTP EAVSVELRPL ILWMANTTEL LSFVQEKVLE MEKEADQEGL SSDPQLCNDL ELCDEALALL DEVIMCTFQQ SVYYLTKTLY STLPALLDSN PFTAGAELPG PGAELEAMPP GLRPTLGVFQ AALELTSQCE LHPDLVSQTF GYLFFFSNAS LLNSLMERGQ GRPFYQWSRA VQIRTNLDLV LDWLQGAGLG DIATEFFRKL SIAVNLLCVP RTSLLKASWS SLRTDYPTLT PAQLHHLLSH YQLGPGRGPP PAWDPPPAER DAVDTGDIFE SFSSHPPLIL PLGSSRLRLT GPVTDDALHR ELRRLRRLLW DLEQQELPAN HRHGPPVAST P
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: RASIP1 (Ras Interacting Protein 1 (RASIP1))

Alternative Name: Rasip1 (RASIP1 Products)

Synonyms: RAIN Protein, 1110025D03Rik Protein, 2610025P08Rik Protein, AI853551 Protein, Rain Protein, Ras interacting protein 1 Protein, RASIP1 Protein, Rasip1 Protein

Background: Ras-interacting protein 1 (Rain),FUNCTION: Required for the proper formation of vascular structures that develop via both vasculogenesis and angiogenesis. Acts as a critical and vascular-specific regulator of GTPase signaling, cell architecture, and adhesion, which is essential for endothelial cell morphogenesis and blood vessel tubulogenesis. Regulates the activity of Rho GTPases in part by recruiting ARHGAP29 and suppressing RhoA signaling and dampening ROCK and MYH9 activities in endothelial cells. May act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS-mediated transformation. Negative regulator of amino acid starvation-induced autophagy (By similarity). {ECO:0000250, ECO:0000269|PubMed:19272373, ECO:0000269|PubMed:21396893}.

Molecular Weight: 103.6 kDa

UniProt: Q3U0S6