PIK3R6 Protein (AA 1-756) (Strep Tag)

Catalog No. ABIN3135024

Product Details

Target: PIK3R6 (Phosphoinositide-3-Kinase, Regulatory Subunit 6 (PIK3R6))

Protein Type: Recombinant

Protein Characteristics: AA 1-756

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This PIK3R6 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MESSDVELDF QRSVQAVLRE LNTPNPALQS NQGMWRWSLH KKVERNPGKS SILVRILLRE LEKAESEDGR RVIIPLLLTL MSVLTKATGI PEDLYHRAYT FCTRLLTLPA PYSTVALDCA IRLKTETAVP GTLYQRTVIA EQNLISELYP YQERVFLFVD PELVSASVCS ALLLEIQAAQ EQQTPEACMR HVVSHALQAA LGEACHTGAL NRKLQASSRR VLEYYFHAVV AAIEQVASED SPSRLGHLEK MEEIYCSLLG PATTRRHCVG DLLQDRLPSI PLPSPYITFH LWTDQEQLWK ELVLFLRPRS QLRLSADLDA LDLQGFRLDR DLARVSTDSG IERDLPLGSD ELPDPSSSEM ERAALQRKGG IKKRVWPPDF FMPGSWDGPP GLHRRTGRPS GDGELLPGVS RVHTARVLVL GDDRMLGRLA QAYYRLRKRE TKKFCLTPRL SLQLYYIPVL APQVTGQDPE ASRKPELGEL ASFLGRVDPW YESTVNTLCP AILKLAEMPP YLDTSRTVDP FILDVITYYV RMGTQPIYFQ LYKVKIFTSL SHDPTEDIFL TELKVKIQDS KSPKEGSSPR RRGAAEGTGA ELSMCYQKAL LSHRPREVTV SLRATGLVLK AIPAGDTEVS GFFHCTSPNA ASATDCSCLH VSVTEVVKSS NLAGRSFTTS TNTFRTSSIQ VQSQDQRLLT LWLDKDGRRT FRDVVRFEVS PCPEPCSRTQ KSKTSALNSH GQETEKNMAK PNSLLMPINT FSGIIQ
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PIK3R6 (Phosphoinositide-3-Kinase, Regulatory Subunit 6 (PIK3R6))

Alternative Name: Pik3r6 (PIK3R6 Products)

Synonyms: C17orf38 Protein, HsT41028 Protein, p84 Protein, p84 PIKAP Protein, p87(PIKAP) Protein, p87PIKAP Protein, BB220380 Protein, RGD1560850 Protein, phosphoinositide-3-kinase regulatory subunit 6 Protein, phosphoinositide-3-kinase regulatory subunit 5 Protein, phosphoinositide-3-kinase, regulatory subunit 6 Protein, PIK3R6 Protein, Pik3r6 Protein

Background: Phosphoinositide 3-kinase regulatory subunit 6 (Phosphoinositide 3-kinase gamma adapter protein of 87 kDa) (p84 PI3K adapter protein) (p84 PIKAP) (p87 PI3K adapter protein) (p87PIKAP),FUNCTION: Regulatory subunit of the PI3K gamma complex. Acts as an adapter to drive activation of PIK3CG by beta-gamma G protein dimers. The PIK3CG:PIK3R6 heterodimer is much less sensitive to beta-gamma G proteins than PIK3CG:PIK3R5 and its membrane recruitment and beta-gamma G protein dimer-dependent activation requires HRAS bound to PIK3CG. Recruits of the PI3K gamma complex to a PDE3B:RAPGEF3 signaling complex involved in angiogenesis, signaling seems to involve RRAS. {ECO:0000269|PubMed:15797027, ECO:0000269|PubMed:16476736, ECO:0000269|PubMed:19906996}.

Molecular Weight: 84.7 kDa

UniProt: Q3U6Q4

Pathways: PI3K-Akt Signaling, Inositol Metabolic Process