TTBK2 Protein (AA 1-1243) (Strep Tag)

Catalog No. ABIN3135103

Product Details

Target: TTBK2 (tau Tubulin Kinase 2 (TTBK2))

Protein Type: Recombinant

Protein Characteristics: AA 1-1243

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This TTBK2 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MSGGGEQPDI LSVGILVKER WKVLRKIGGG GFGEIYDALD MLTRENVALK VESAQQPKQV LKMEVAVLKK LQGKDHVCRF IGCGRNDRFN YVVMQLQGRN LADLRRSQSR GTFTISTTLR LGKQILESIE SIHSVGFLHR DIKPSNFAMG RFPSTCRKCF MLDFGLARQF TNSCGDVRPP RAVAGFRGTV RYASINAHRN REMGRHDDLW SLFYMLVEFV VGQLPWRKIK DKEQVGSIKE RYDHRLMLKH LPPEFSTFLD HISSLDYFTK PDYQLLTSVF DNSIKTFGVI ESDPFDWEKS GTDGSLTTTT TSATPQLHTR LTPAAIGIAN ATPIPGDLLR ENTDEVFPDE QLSDGENGIP VGVSPDKLPG SLGHPRPQEK DVWEEMDINK NKIKLGICKA ATEEENSHGQ VNGILNAPSL GSPIRVRSEI TQPDRDVPLV RKLRSIHSFE LEKRLTLEPK PDTDKFLETC MEKMQKDSSA GKEPVPPALP HKPCVPVVTH TDHIWHYDDE YLPDASKPAS ANTPEQADGG GSNGFIAVNL SSCKQEVDSK EWVIVDKEQD LQDFRTNEVL GHKTTGSPSD EEPEVLQVLE GSPQDEKIQV GPWTDNHHLK KESSGVVLAL SAECPATAAS ELYTDRLDLQ AGAASQFITV TPTSPMEAQA EGPLTAITIP RPSVASTQST SGSFHYGPQP EKKDLQPLEP TVELYSPREN FSGLVVTEGE LASGGSRVDL GLQIDHTGHD MLPNMRDGDT SQDLGPKDPP DHNRLAVKEF EHLPGETEER SLLLGSENED ERLSKGQHCI EVSSPGELVT AERAQLAATE PLHVSETQNC SVLPNQDKTH EIMKLLAVGT SEISPQAIDP HAEGQIGQMA AMQKNKLFKD DGIQSESLPR QQGDLSAFLH QEGKREKVVP RNGELYHCVS ENEHGPPTRK DMLRSSFVTR HSRIPVLAQE IDSTFESSSA ISAKEKLLQK KAYQPEIVKL LVEKRQFKSF LGDLSSASDK LIEEKLAAVP VPFSEEEVFA PFSRLAADSH LSRSVEDSFL SPIISQARKS KIPRPVSWVS TDQINGSASP QFLPRPPPGK PPVRPGVEAR LRRYKVLGSS NSDSDLFSRL AQILQNGSQK SRSTTQCKSP GSPHNPKTPP KSPVVPRRSP SASPRSSSLP RTSSSSPSRA GRPHHDQRSS SPHLGRSKSP PSHSGSSSSR RSCQQEHCKP SKNGPKGSGS LHHHSTSSKT PPGKSKPASK LSR
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: TTBK2 (tau Tubulin Kinase 2 (TTBK2))

Alternative Name: Ttbk2 (TTBK2 Products)

Synonyms: ttbk Protein, MGC146331 Protein, 2610507N02Rik Protein, AI326283 Protein, B930008N24Rik Protein, TTK Protein, Ttbk Protein, Ttbk1 Protein, mKIAA0847 Protein, SCA11 Protein, TTBK Protein, tau tubulin kinase 2 Protein, TTBK2 Protein, ttbk2 Protein, Ttbk2 Protein

Background: Tau-tubulin kinase 2 (EC 2.7.11.1) (Protein bartleby),FUNCTION: Serine/threonine kinase that acts as a key regulator of ciliogenesis: controls the initiation of ciliogenesis by binding to the distal end of the basal body and promoting the removal of CCP110, which caps the mother centriole, leading to the recruitment of IFT proteins, which build the ciliary axoneme. Has some substrate preference for proteins that are already phosphorylated on a Tyr residue at the +2 position relative to the phosphorylation site. Able to phosphorylate tau on serines in vitro (PubMed:23141541). Phosphorylates MPHOSPH9 which promotes its ubiquitination and proteasomal degradation, loss of MPHOSPH9 facilitates the removal of the CP110-CEP97 complex (a negative regulator of ciliogenesis) from the mother centrioles, promoting the initiation of ciliogenesis (By similarity). {ECO:0000250|UniProtKB:Q6IQ55, ECO:0000269|PubMed:11257498, ECO:0000269|PubMed:23141541}.

Molecular Weight: 136.8 kDa

UniProt: Q3UVR3