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Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE) (AA 183-1167) protein (rho-1D4 tag) Protein

ITGAE Origin: Mouse Source: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Catalog No. ABIN3135283
$7,307.36
Plus shipping costs $45.00 and $22.00 dry ice
0.25 mg ABIN3135283
0.25 mg ABIN3135283
local_shipping Shipping to: United States
Delivery in 60 to 65 Business Days
  • Protein Name
    Protein Type
    Recombinant
    Protein Characteristics
    AA 183-1167
    Origin
    Mouse
    Source
    Insect Cells
    Purification tag / Conjugate
    This ITGAE protein is labelled with rho-1D4 tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    TVEEEDEEED GTEIAIVLDG SGSIEPSDFQ KAKNFISTMM RNFYEKCFEC NFALVQYGAV IQTEFDLQES RDINASLAKV QSIVQVKEVT KTASAMQHVL DNIFIPSRGS RKKALKVMVV LTDGDIFGDP LNLTTVINSP KMQGVVRFAI GVGDAFKNNN TYRELKLIAS DPKEAHTFKV TNYSALDGLL SKLQQHIVHM EGTVGDALQY QLAQTGFSAQ ILDKGQVLLG TVGAFNWSGG ALLYSTQNGR GCFLNQTAKE DSRTVQYSYL GYSLAVLHKA HGVSYVAGAP RHKLRGAVFE LRKEDREEDA FVRRIEGEQM GSYFGSVLCP VDIDMDGTTD FLLVAAPFYH IRGEEGRVYV YQVPEQDASF SLAHTLSGHP GLTNSRFGFA MAAVGDINQD KFTDVAIGAP LEGFGAGDGA SYGSVYIYNG HSGGLYDSPS QQIRASSVAS GLHYFGMSVS GGLDFNGDGL ADITVGSRDS AVVLRSRPVV DLTVSMTFTP DALPMVFIGK MDVKLCFEVD SSGVASEPGL REMFLNFTVD VDVTKQRQRL QCEDSSGCQS CLRKWNGGSF LCEHFWLIST EELCEEDCFS NITIKVTYEF QTSGGRRDYP NPTLDHYKEP SAIFQLPYEK DCKNKVFCIA EIQLTTNISQ QELVVGVTKE VTMNISLTNS GEDSYMTNMA LNYPRNLQFK KIQKPVSPDV QCDDPKPVAS VLVMNCKIGH PILKRSSVNV SVTWQLEESV FPNRTADITV TISNSNEKSL ARETRSLQFR HAFIAVLSRP SVMYMNTSQS PSDHKEFFFN VHGENLFGAV FQLQICVPIK LQDFQIVRVK NLTKTQDHTE CTQSQEPACG SDPVQHVKEW HSVVCAITSN KENVTVAAEI SVGHTKQLLR DVSELPILGE ISFNKSLYEG LNAENHRTKI TVIFLKEEET RSLPLIIGSS IGGLLVLVVI IAILFKCGFF KRKYQQLNLE STRRAQLKAD SLLQD
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Itgae Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin-free.
    Grade
    Crystallography grade
  • Protein Name
    Background
    Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers. Mice expressing a null mutation of the alpha-E subunit gene exhibit a marked reduction in the numbers of intraepithelial lymphocytes in the gut and in the development of gut-associated lymphoid aggregates, supporting a specific role for this integrin in mediating retention of lymphocytes in the intestinal wall.
    Molecular Weight
    110.2 kDa Including tag.
    UniProt
    Q60677
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
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