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MERTK Protein (AA 519-994) (His tag)

Crystallography grade MERTK Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Catalog No. ABIN3135307
  • Target See all MERTK Proteins
    MERTK (C-Mer Proto-Oncogene Tyrosine Kinase (MERTK))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 519-994
    Origin
    • 22
    • 7
    • 3
    • 1
    • 1
    Mouse
    Source
    • 13
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This MERTK protein is labelled with His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequence
    ALRRRVQETK FGGAFSEEDS QLVVNYRAKK SFCRRAIELT LQSLGVSEEL QNKLEDVVID RNLLVLGKVL GEGEFGSVME GNLKQEDGTS QKVAVKTMKL DNFSQREIEE FLSEAACMKD FNHPNVIRLL GVCIELSSQG IPKPMVILPF MKYGDLHTFL LYSRLNTGPK YIHLQTLLKF MMDIAQGMEY LSNRNFLHRD LAARNCMLRD DMTVCVADFG LSKKIYSGDY YRQGRIAKMP VKWIAIESLA DRVYTSKSDV WAFGVTMWEI TTRGMTPYPG VQNHEMYDYL LHGHRLKQPE DCLDELYDIM YSCWSADPLD RPTFSVLRLQ LEKLSESLPD AQDKESIIYI NTQLLESCEG IANGPSLTGL DMNIDPDSII ASCTPGAAVS VVTAEVHENN LREERYILNG GNEEWEDVSS TPFAAVTPEK DGVLPEDRLT KNGVSWSHHS TLPLGSPSPD ELLFVDDSLE DSEVLM
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Mertk Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product MERTK Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    MERTK (C-Mer Proto-Oncogene Tyrosine Kinase (MERTK))
    Alternative Name
    Mertk (MERTK Products)
    Synonyms
    MER Protein, RP38 Protein, c-mer Protein, Eyk Protein, Mer Protein, Nyk Protein, nmf12 Protein, rdy Protein, C-EYK Protein, MERTK Protein, DKFZp469D2215 Protein, si:ch1073-268l12.2 Protein, MER proto-oncogene, tyrosine kinase Protein, c-mer proto-oncogene tyrosine kinase Protein, c-mer proto-oncogene tyrosine kinase a Protein, MERTK Protein, Mertk Protein, mertka Protein
    Background
    Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. {ECO:0000269|PubMed:19117932, ECO:0000269|PubMed:20363878}.
    Molecular Weight
    54.7 kDa Including tag.
    UniProt
    Q60805
    Pathways
    RTK Signaling
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