INSM1 Protein (AA 1-521) (Strep Tag)

Catalog No. ABIN3135567

Product Details

Target: INSM1 (Insulinoma-Associated 1 (INSM1))

Protein Type: Recombinant

Protein Characteristics: AA 1-521

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This INSM1 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MPRGFLVKRS KKSTPVSYRV RGGEDSDRAL LLSPGCGGAR AEPPVPSPGP LPPPPPPALA ERAHAALAAA LACAPGPPPP PPPGPRAAHF GNPEAAHPAP LYSPTRPVSR EHEKHKYFER SFNLGSPVSA ESFPTPAALL AGGGSGANGA GGGGGGTCGG DALLFAPAEL KMGTAFSAGA EAARGPGTGP PLSPAAALRP PGKRPAPPAA VATEPPAKAA KAPSAKKPKA IRKLHFEDEV TTSPVLGLKI KEGPVEAPRG RAGGATRPLG EFICQLCKEE YADPFALAQH KCSRIVRVEY RCPECAKVFS CPANLASHRR WHKPRPVPAA ARAPEPEAAT RAEAREAAGG GSSDRDTPSP GGVSESGSED GLYECHHCAK KFRRQAYLRK HLLAHHQALQ AKGAPPPPPP PPPPAEDILA FYAGPDEKAP QEASGDGEAA GVLGLSATAQ CHLCPVCGET FPSKGAQERH LRLLHAAQVF PCKYCPATFY SSPGLTRHIN KCHPSENRQV ILLQVPVRPA C
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: INSM1 (Insulinoma-Associated 1 (INSM1))

Alternative Name: Insm1 (INSM1 Products)

Synonyms: IA-1 Protein, IA1 Protein, INSM-1 Protein, Insm1 Protein, fc13a09 Protein, wu:fc13a09 Protein, zINSM-1 Protein, zgc:63866 Protein, INSM1 Protein, ia-1 Protein, zgc:77257 Protein, INSM transcriptional repressor 1 Protein, insulinoma-associated 1 Protein, insulinoma-associated 1b Protein, insulinoma associated 1 Protein, insulinoma associated 1 L homeolog Protein, insulinoma-associated 1a Protein, INSM1 Protein, Insm1 Protein, insm1b Protein, insm1 Protein, insm1.L Protein, insm1a Protein

Background: Insulinoma-associated protein 1 (Zinc finger protein IA-1),FUNCTION: Sequence-specific DNA-binding transcriptional regulator that plays a key role in neurogenesis and neuroendocrine cell differentiation during embryonic and/or fetal development. Binds to the consensus sequence 5'-[TG][TC][TC][TT][GA]GGG[CG]A-3' in target promoters. Acts as a transcriptional repressor of NEUROD1 and INS expression via its interaction with cyclin CCND1 in a cell cycle-independent manner. Negatively regulates skeletal muscle-specific gene expression in endocrine cells of the pituitary by inhibiting the Notch signaling pathway. Represses target gene transcription by recruiting chromatin-modifying factors, such as HDAC1, HDAC2, HDAC3, KDM1A and RCOR1 histone deacetylases. Binds to its own promoter, suggesting autoregulation as a self-control feedback mechanism. Competes with histone H3 for the same binding site on the histone demethylase complex formed by KDM1A and RCOR1, and thereby inhibits demethylation of histone H3 at 'Lys-4' (By similarity). Promotes the generation and expansion of neuronal basal progenitor cells in the developing neocortex. Involved in the differentiation of endocrine cells of the developing anterior pituitary gland, of the pancreas and intestine, and of sympatho-adrenal cells in the peripheral nervous system. Promotes cell cycle signaling arrest and inhibition of cellular proliferation. {ECO:0000250|UniProtKB:Q01101, ECO:0000269|PubMed:16511571, ECO:0000269|PubMed:16951258, ECO:0000269|PubMed:18094025, ECO:0000269|PubMed:18940587, ECO:0000269|PubMed:24227653}.

Molecular Weight: 54.1 kDa

UniProt: Q63ZV0