PTPRJ Protein (AA 29-876) (His tag)

Catalog No. ABIN3135603

Product Details

Target: PTPRJ (Protein tyrosine Phosphatase, Receptor Type, J (PTPRJ))

Protein Type: Recombinant

Protein Characteristics: AA 29-876

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This PTPRJ protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: RQGQVLCAGA APNPIFDIEA VVSPTSVLLT WKHNDSGASE CRIENKMESN LTFPVKNQTS CNITGLSPGT SYTFSIISVT TNETLNKTIT TEPWPVSDLH VTSVGVTQAR LTWSNANGTA SYRMLIEELT THSSVNISGL KPGTNNSFAF PESNETQADF AVAEEVPDAN GTKRIPVTNL SQLHKNSLVS VDPPSGQDPS LTEILLTDLK PDTQYNATIY SQAANGTEGQ PRNKVFKTNS TQVSDVRAMN ISASSMTLTW KSNYDGSRTS IVYKIHVAGG THSVNQTVNK TEAIILGLSS STLYNITVHP FLGQTEGTPG FLQVYTSPDQ VSDFRVTNVS TRAIGLAWRS NDSKSFEIFI KQDGGEKHRN ASTGNQSYMV EDLKPGTSYH FEIIPRGPDG TEGLSSTVNG STDPSAVTDI RVVNISTTEM QLEWQNTDDA SGYTYHLVLE SKSGSIIRTN SSQKWITVGS LTPGTLYNVT IFPEVDQIQG ISNSITQYTR PSSVSHIEVN TTTTTAAIRW KNEDAASASY AYSVLILKTG DGSNVTSNFT KDPSILIPEL IPGVSYTVKI LTQVGDGTTS LVPGWNLFCT EPEPVTSFHC EVVPKEPALV LKWACPFGMY TGFELGVRSD SWDNMTRLEN CTSDDDTECR TEVAYLNFST SYNISIATLS CGKMALPAQN ICTTGITDPP TPDGSPNITS VSHNSVKVKF SGFEASHGPI KAYAVILTTG EAAQPSADVL KYTYEDFKRG ASDTYVTYLI RIEEKGQSQG LSEVLNYEID VGNQSTTLGY YNGRLEPLGS YRACVAGFTN ITYNLQNDGL INGDESYVSF SPYSEAVFLP QDPGVICG
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Ptprj Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PTPRJ (Protein tyrosine Phosphatase, Receptor Type, J (PTPRJ))

Alternative Name: Ptprj (PTPRJ Products)

Synonyms: HAAP Protein, CD148 Protein, DEP1 Protein, HPTPeta Protein, R-PTP-ETA Protein, SCC1 Protein, AI450271 Protein, BET Protein, Byp Protein, DEP-1 Protein, PTPbeta2 Protein, Ptpb2 Protein, RPTPJ Protein, Scc-1 Protein, Scc1 Protein, PTPRJ Protein, acid phosphatase 1 Protein, protein tyrosine phosphatase, receptor type J Protein, protein tyrosine phosphatase, receptor type, J Protein, ACP1 Protein, PTPRJ Protein, Ptprj Protein, EUBREC_2762 Protein

Background: Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, FLT3, PDGFRB, MET, RET, KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Involved in vascular development. May be involved in the mechanism of contact inhibition of cell growth. Regulator of macrophage adhesion and spreading. Positively affects cell-matrix adhesion. Positive regulator of platelet activation and thrombosis. Negative regulator of cell proliferation. Negative regulator of PDGF-stimulated cell migration, through dephosphorylation of PDGFR. Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation, through KDR dephosphorylation. Negative regulator of EGFR signaling pathway, through EGFR dephosphorylation. Enhances the barrier function of epithelial junctions during reassembly. Negatively regulates T-cell receptor (TCR) signaling. Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling. {ECO:0000269|PubMed:12588999, ECO:0000269|PubMed:12771128, ECO:0000269|PubMed:12833140, ECO:0000269|PubMed:12913111, ECO:0000269|PubMed:18249142, ECO:0000269|PubMed:19246339, ECO:0000269|PubMed:19268662}.

Molecular Weight: 92.8 kDa Including tag.

UniProt: Q64455

Pathways: EGFR Signaling Pathway, Platelet-derived growth Factor Receptor Signaling