PLEKHG5 Protein (AA 1-1073) (Strep Tag)

Catalog No. ABIN3135618

Product Details

Target: PLEKHG5 (Pleckstrin Homology Domain Containing, Family G (With RhoGef Domain) Member 5 (PLEKHG5))

Protein Type: Recombinant

Protein Characteristics: AA 1-1073

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This PLEKHG5 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MGTGPGVSGR RAAARPSSEL PSPDSQLLWV GGHAHSSDSQ VCHHADCQQL HHRGPLNLCE TCDSKFHSTL HYDGHVRFDL PPQGSVLARN VSTRSCPPRT SPAADLEEEE EGCTDGKGDR KSAGLKISKK KARRRHTDDP SKECFTLKFD LNVDIETEIV PAMKKKSLGE VLLPVFERKG IALGKVDIYL DQSNTPLSLT FEAYRFGGHY LRVKAKPGDE GKVEQGVKDS KSLSLPALRP SGAGPPVSER VDPQSRRESS LDILAPGRRR KNMSEFLGEA GIPGHEPPAP SSCSLPVGSS GGTSSGINES WKNRAASRFS GFFSSSPSTS AFSREVDKME QLESKLHAYS LFGLPRMPRR LRFDHDSWEE EEEDDEEDEE SSGLRLEDSW RELTDGHEKL TRRQCHQQEA VWELLHTEVS YIRKLRVITN LFLCCLLNLQ ESGLLCEVEA ERLFSNIPEI AKLHRGLWGS VMVPVLEKAR RTRALLQPSD FLKGFKMFGS LFKPYIRYCM EEEGCMEYMR GLLRDNDLFR AYVTWAEKHQ QCQRLKLSDM LAKPHQRLTK YPLLLKSVLR KTDDPRTKEA IVTMISSVER FIHHVNTCMR QRQERQRLAG VVSRIDAYEV VEGSNDEVDK LLKEFLHLDL TAPMPGTSPE ETRQLLLEGS LRMKEGKDSK MDVYCFLFTD LLLVTKAVKK AERTKVIRPP LLVDKIVCRE LRDPGSFLLI YLNEFHSAVG AYTFQASSQA LCRSWVDTIY NAQNQLQQLR AQLSAQEHPG SQHLQSLEEE EDEQEEEGEE SGTSAASSPT ILRKSSNSLD SEHCTSDGST ETLAMVVVEP GATLSSPEFE GGPVSSQSDE SSLSNTASSV TPTSELLPLG PVDGRSCSMD SAYGTLSPTS LQDFVAPHPV VEPAPVPQTP SPQPSPRLRR RTPVQLLPRP PRLLKSKSEA SLLQLLSGTP AARGVPPAPS RSLSELCLIS VAPGVRTQRP LQEGGPGWNG PGMCDPCHGP QLSESENRPS HMTGGPADSA RRRCREMPSG TMSRVQSEPP SGVSAQHRKL TLAQLYRIRT TLLLNSTLTA SEV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PLEKHG5 (Pleckstrin Homology Domain Containing, Family G (With RhoGef Domain) Member 5 (PLEKHG5))

Alternative Name: Plekhg5 (PLEKHG5 Products)

Synonyms: PLEKHG5 Protein, plekhg5 Protein, si:ch211-15p9.7 Protein, syx Protein, syx-a Protein, DSMA4 Protein, GEF720 Protein, Syx Protein, Tech Protein, BC023181 Protein, mKIAA0720 Protein, pleckstrin homology and RhoGEF domain containing G5 Protein, pleckstrin homology domain containing, family G (with RhoGef domain) member 5a Protein, pleckstrin homology domain containing, family G (with RhoGef domain) member 5 Protein, PLEKHG5 Protein, plekhg5a Protein, plekhg5 Protein, Plekhg5 Protein

Background: Pleckstrin homology domain-containing family G member 5 (PH domain-containing family G member 5) (Synectin-binding RhoA exchange factor) (SYX),FUNCTION: Functions as a guanine exchange factor (GEF) for RAB26 and thus regulates autophagy of synaptic vesicles in axon terminal of motoneurons (PubMed:29084947). Involved in the control of neuronal cell differentiation. Plays a role in angiogenesis through regulation of endothelial cells chemotaxis (PubMed:21543326). Affects also the migration, adhesion, and matrix/bone degradation in macrophages and osteoclasts (By similarity). {ECO:0000250|UniProtKB:O94827, ECO:0000269|PubMed:21543326, ECO:0000269|PubMed:29084947}.

Molecular Weight: 118.9 kDa

UniProt: Q66T02

Pathways: Neurotrophin Signaling Pathway