FNIP1 Protein (AA 1-1165) (Strep Tag)

Catalog No. ABIN3135629

Product Details

Target: FNIP1 (Folliculin Interacting Protein 1 (FNIP1))

Protein Type: Recombinant

Protein Characteristics: AA 1-1165

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This FNIP1 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MAPTLFQKLF SKRSGLGAPG RDARDPDCAF SWPLPEFDPS QIRLIVYQDC ERRGRNVLFD SSVKRKNEDT SVSKLCNDAQ VKVFGKCCQL KPGGDSSSSL DSSITLSSDG KDQCPKYQGS RCSSDANMLG EMMFGSVAMS YKGSTLKIHQ IRSPPQLMLS KVFTARTGSS ICGSLNTLQD SLEFINQDSN TLKADSSTVS NGLLGNIGLS QFCSPRRAFS EQGPLRLIRS ASFFAVHSNP MDMPGRELNE DRDSGIARSA SLSSLFITPF PSPNSSLTRS CASSYQRRWR RSQTTSLENG VFPRWSVEES FNLSDESCGP NPGIVRKKKI AIGVIFSLSK DEDENNKFNE FFFSHFPLFE SHMNKLKSAI EQAMKMSRRS ADASQRSLAY NRILDALTEF RTTICNLYTM PRIGEPVWLT MMSGTPEKNQ LCHRFMKEFT FLMENASKNQ FLPALITAVL TNHLAWVPTV MPNGQPPIKI FLEKHSSQSV GMLAKTHPYN PLWAQLGDLY GAIGSPVRLA RTVVVGKRQD LVQRLLYFLT YFIRCSELQE THLLENGEDE AIVMPGTVIT TTLEKGEIEE SEYVLITMHR NKSSLLFKES EETRTPNCNC KYCSHPVLGQ NTENVSQPER EDTQDNSKEL LGISDECQKI SPPDCQEENA VDVQQYRDKL RTCLDTKLET VVCTGSAPAD KCVLSETCLE PREESWQNKE LLDSDNHTGT AMRPTGIVVE KKPPDKNVPS AFSCEVTQTK VTFLIGDSMS PDSDTELRSQ AVVDQINRHH SEPLKEDRGV ADKHQESKIT KDQSEDSDTQ NIVSGESCEL PCWSHSDPES MSLFDEYFND DSIETRTIDD VPVKTSTDSK EYCCMLEYPK RLYTKTNKQK SELCKCIETV HQDSCNACFP QQDQRNSLSI LVPHGDKESS DKKNAVGTEW DIPRNESSDS ALGDSESEDT GPDIRRQAGG YCGGDQEDWT EEDEIPFPGS KLIEVSAVQP NIANFGRSLL GGYCSSYVPD FVLQGIGNDE RLRQCLVSDL SHAVQHPVLD EPIAEAVCII ADMDKWTVQV ASSQRRVTDN KLGKEVLVSS LVSNLLHSTL QLYKHNLSPN FCVMHLEDRL QELYFKSKML SEYLRGQMRV HVKELGVVLG IESSDLPLLA AVASTHSPYV AQILL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: FNIP1 (Folliculin Interacting Protein 1 (FNIP1))

Alternative Name: Fnip1 (FNIP1 Products)

Synonyms: FNIP1 Protein, DKFZp468P0310 Protein, A730024A03Rik Protein, AI838773 Protein, AW557298 Protein, folliculin interacting protein 1 Protein, FNIP1 Protein, fnip1 Protein, Fnip1 Protein

Background: Folliculin-interacting protein 1,FUNCTION: Binding partner of the GTPase-activating protein FLCN: involved in the cellular response to amino acid availability by regulating the non-canonical mTORC1 signaling cascade controlling the MiT/TFE factors TFEB and TFE3 (PubMed:23582324). Required to promote FLCN recruitment to lysosomes and interaction with Rag GTPases, leading to activation of the non-canonical mTORC1 signaling (By similarity). In low-amino acid conditions, component of the lysosomal folliculin complex (LFC) on the membrane of lysosomes, which inhibits the GTPase-activating activity of FLCN, thereby inactivating mTORC1 and promoting nuclear translocation of TFEB and TFE3 (By similarity). Upon amino acid restimulation, disassembly of the LFC complex liberates the GTPase-activating activity of FLCN, leading to activation of mTORC1 and subsequent inactivation of TFEB and TFE3 (By similarity). Together with FLCN, regulates autophagy: following phosphorylation by ULK1, interacts with GABARAP and promotes autophagy (By similarity). In addition to its role in mTORC1 signaling, also acts as a co-chaperone of HSP90AA1/Hsp90: following gradual phosphorylation by CK2, inhibits the ATPase activity of HSP90AA1/Hsp90, leading to activate both kinase and non-kinase client proteins of HSP90AA1/Hsp90 (By similarity). Acts as a scaffold to load client protein FLCN onto HSP90AA1/Hsp90 (By similarity). Competes with the activating co-chaperone AHSA1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (By similarity). Also acts as a core component of the reductive stress response by inhibiting activation of mitochondria in normal conditions: in response to reductive stress, the conserved Cys degron is reduced, leading to recognition and polyubiquitylation by the CRL2(FEM1B) complex, followed by proteasomal (PubMed:32941802). Required for B-cell development (PubMed:22709692, PubMed:27303042). {ECO:0000250|UniProtKB:Q8TF40, ECO:0000250|UniProtKB:Q9P278, ECO:0000269|PubMed:22709692, ECO:0000269|PubMed:23582324, ECO:0000269|PubMed:27303042, ECO:0000269|PubMed:32941802}.

Molecular Weight: 130.1 kDa

UniProt: Q68FD7