MYSM1 Protein (AA 1-819) (Strep Tag)

Catalog No. ABIN3135641

Product Details

Target: MYSM1 (Myb-Like, SWIRM and MPN Domains 1 (MYSM1))

Protein Type: Recombinant

Protein Characteristics: AA 1-819

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This MYSM1 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MEAEEADVDV EGDVAAAAQP GNDESTASVF QDHYLDSTWR RENGCLPWTL DSTISDENRA IIEKMLLEEE YYLSNKSLPG KFWVNQKEDN KKYTNSLQKS SKAMVDSPAK PASHSVKWTV EEKELFEQGL AKFGRRWTKI ATLLKSRTVL QVKSYARQYF KNKVKWDVEK ETPTQKSSSD LQVKNKDDRT KAWAAACLRG SADPCLNAVK IEKLSDDEDV DITDELDELT SQTSQNSGSH LTLDVPNSKM YTTNQGELCQ EGPLAKSSGE SLQNVKQGEG EACSSSEIAS WAEKQKSTDK NSAELNEKYN KVVEEHTLHR GEVREEAKHS PSPEPCERQD SSGNEMLLPP CQIEEENHEG EELKPPEQEV EIDRNVIQEE EKQAIPEFFE GRQTKTPERY LKIRNYILDQ WEICKPKYLN KTSVRPGLKN CGDVNCIGRI HTYLELIGAI NFGCEQAVYN RPQPLDKVRA ADRKDAEAAY QLAWRLQSMR TRRRRVRDPW GNWCDAKDLE GQTFEHLSVE EMARRKEEEK CKPIKFSKAS KLPKSSLDPF QLIPCNFFSE EKQEPFQVKV AAEALLIMNL HAHVSMAEVI GLLGGRYSEA DKVLEVCAAE PCNSLSTGLQ CEMDPVSQTQ ASETLALRGY SVIGWYHSHP AFDPNPSLRD IDTQAKYQSY FSRGGAKFIG MIVSPYNRSN PLPYSQITCL VISEEVSPDG TYRLPYKFEV QQMLEEPQWE LVFEKTRWII EKYRLSNSSV PMDRIFRRDS DLTCLQKLLE CLRKTLSKVA NCFIAEEFLT QIENLFLSNY KSKEENGLAE EDSTKELFM
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: MYSM1 (Myb-Like, SWIRM and MPN Domains 1 (MYSM1))

Alternative Name: Mysm1 (MYSM1 Products)

Synonyms: 2A-DUB Protein, 2ADUB Protein, RP4-592A1.1 Protein, C130067A03Rik Protein, C530050H10Rik Protein, RGD1311787 Protein, 2adub Protein, Myb like, SWIRM and MPN domains 1 Protein, myb-like, SWIRM and MPN domains 1 Protein, Myb like, SWIRM and MPN domains 1 L homeolog Protein, MYSM1 Protein, Mysm1 Protein, mysm1.L Protein

Background: Deubiquitinase MYSM1 (2A-DUB) (EC 3.4.19.-) (Myb-like, SWIRM and MPN domain-containing protein 1),FUNCTION: Metalloprotease with deubiquitinase activity that plays important regulator roles in hematopoietic stem cell function, blood cell production and immune response (PubMed:26474655, PubMed:27895164, PubMed:30405132). Participates in the normal programming of B-cell responses to antigen after the maturation process (PubMed:27895164). Within the cytoplasm, plays critical roles in the repression of innate immunity and autoimmunity (PubMed:26474655, PubMed:30405132). Removes 'Lys-63'-linked polyubiquitins from TRAF3 and TRAF6 complexes (PubMed:26474655). Attenuates NOD2-mediated inflammation and tissue injury by promoting 'Lys-63'-linked deubiquitination of RIPK2 component (PubMed:30405132). Suppresses the CGAS-STING1 signaling pathway by cleaving STING1 'Lys-63'-linked ubiquitin chains (PubMed:33086059). In the nucleus, acts as a hematopoietic transcription regulator derepressing a range of genes essential for normal stem cell differentiation including EBF1 and PAX5 in B-cells, ID2 in NK-cell progenitor or FLT3 in dendritic cell precursors (PubMed:22169041, PubMed:24062447, PubMed:25217698, PubMed:26348977). Deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, leading to dissociation of histone H1 from the nucleosome (By similarity). {ECO:0000250|UniProtKB:Q5VVJ2, ECO:0000269|PubMed:22169041, ECO:0000269|PubMed:24062447, ECO:0000269|PubMed:25217698, ECO:0000269|PubMed:26348977, ECO:0000269|PubMed:26474655, ECO:0000269|PubMed:27895164, ECO:0000269|PubMed:30405132}.

Molecular Weight: 93.5 kDa

UniProt: Q69Z66

Pathways: Chromatin Binding