EFCAB6 Protein (AA 1-1516) (Strep Tag)

Catalog No. ABIN3135735

Product Details

Target: EFCAB6 (EF-Hand Calcium Binding Domain 6 (EFCAB6))

Protein Type: Recombinant

Protein Characteristics: AA 1-1516

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This EFCAB6 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MKRNGTRLNF AKANSTKSGS TRARDLMNKM SIIPEWHSLY SNTRKLPCSR PHSSPCKMQR TIFQDLCRPS SSTTAIANPV LSYLDIERIL AQKISSRRDD IKKVFQILDR NHNQMVTKGD LKRVITAFLI PLTKDQFQDL LAQIPISSLG NVPYLEFLSR FGGGIDININ GIKRVNENEV DNRRTVKEVQ LTEKIFRNMR SIRKVFQVMD VNNTGLVQPQ ELRRVLETFC LRMQDGDYEK FLEQYNIDKT TAVDYNAFLK NLSVKNDASF KYLLSNAAEL SRETQQGKNG KRLLDTGSSE DVWKNYSLDD LEKTFCQEFS KSYEKIEKAL SAGDPSKGGY ISLNYLKVVL DTFIYRLPRR IFIQLIRRFG LKTSTKINWK QFLTAIYERQ KLEVSKTLPL KKRSSTEARN RSRKENIIKK LFKYSEDRYT ALKKTLLIIS STPSGHITWE ELRHILNCMV AKLNDSEFSE LKQTFDPEGT GAVRVNSLLD VLDDSTKVRK MSPSTDTKTP LPVAWDSVEE LVLDSITRNL QAFYSMLQSY DLRDTGTIGK NNFRKVMRVF CPYLSNEHLV RFSSKFQEAG SGRILYKKFL LSIGVGIPPP TPPLSSPKVQ LSDQFQIEEP GQQDERTQPS GEKTSEINNM TKEEVIDGLK HRIQQKDPVF RKQFLSISKE PDVKINQEEF RKVLERSGMP MNDCQYAMLA SKLGFKNEEG MSYQDFTMGF EDCMLSGLET VPLQSRTASR TNMDEHFISA EECLRIFPKK LKESFRDVYS AFFRIDLDRD GIISMHDFHR LLQYLQLNMV DLEFERFLSL LGLRLSVTLN FREFQNLCEK RPWKSDEAPQ RLIRCKQKVA DSELACEQAH QYLIMKAKTR WADLSKNFIE TDNEGNGILR RRDIKNSLYG FDIPLTPREF EKLWQNYDTE GRGYITYQEF LHRLGIRYSP KVHRPYKEDY FNFLGHFTKP KQVQEEIQEL QQISEREKLM NHYEEISKAF NAMEKSKPVA LCRVQKVLQE CGCPLKEEEL ISLLKSLDVS VHNNHIDPVE FLRALEISWA SKARPKEKEE SSPPPISFSK VTPDEVIKTM QEVVESSQPA LVEAFSALDK EDTGFVKAME FGDVLRSVCQ KLTDNQYHYF LRRLRLHLTP NIHWKYFLEN FSTFQDETAD DWAENMPKAP PPMSPKETAH RDIVARVQKA VASHYHTIVQ EFENFDTLKS NTVSRDEFRS ICTRHIQILT DEQFDRLWSE LPVNAKGRLK YQDFLSKLSI ERVPSPPMAA GDSGESTMAQ RGSSAPEFSQ GTRSNLYSPP RDSRVGLKSR SHPCTPVGTP PLQNCEPIES RLRKQIQGCW RELLRECKEK DTDKQGTISA AEFLALVEKF KLDISREESQ QLIVKYDLKN NGKFAYCDFI QSCVLLLKAK ETSLMRRMRI QNADKMKEAG METPSFYSAL LRIQPKIVHC WRPMRRSFKT YDKNGTGLLS VADFRKVLRQ YSINLSEEEF FHVLEYYDKS LSSKISYNDF LRAFLQ
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: EFCAB6 (EF-Hand Calcium Binding Domain 6 (EFCAB6))

Alternative Name: Efcab6 (EFCAB6 Products)

Synonyms: DJBP Protein, HSCBCIP1 Protein, dJ185D5.1 Protein, 4931407K02Rik Protein, 4932408N08Rik Protein, bM150J22.2 Protein, mKIAA1672 Protein, EF-hand calcium binding domain 6 Protein, EFCAB6 Protein, Efcab6 Protein

Background: EF-hand calcium-binding domain-containing protein 6 (DJ-1-binding protein) (DJBP),FUNCTION: Negatively regulates the androgen receptor by recruiting histone deacetylase complex, and protein DJ-1 antagonizes this inhibition by abrogation of this complex. Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating. {ECO:0000250|UniProtKB:Q5THR3}.

Molecular Weight: 175.8 kDa

UniProt: Q6P1E8