KDM2B Protein (AA 1-1309) (Strep Tag)

Catalog No. ABIN3135737

Product Details

Target: KDM2B (Lysine (K)-Specific Demethylase 2B (KDM2B))

Protein Type: Recombinant

Protein Characteristics: AA 1-1309

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This KDM2B protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MEAEKDSGRR LRAIDRQRYD ENEDLSDVEE IVSVRGFSLE EKLRSQLYQG DFVHAMEGKD FNYEYVQREA LRVPLVFRDK DGLGIKMPDP DFTVRDVKLL VGSRRLVDVM DVNTQKGTEM SMSQFVRYYE TPEAQRDKLY NVISLEFSHT KLEHLVKRPT VVDLVDWVDN MWPQHLKEKQ TEATNALAEM KYPKVKKYCL MSVKGCFTDF HIDFGGTSVW YHVFRGGKIF WLIPPTLHNL ALYEEWVLSG KQSDIFLGDR VERCQRIELK QGYTFFIPSG WIHAVYTPVD SLVFGGNILH SFNVPMQLRI YEIEDRTRVQ PKFRYPFYYE MCWYVLERYV YCVTQRSYLT QEYQRELMLI DAPRKTSVDG FSSDSWLDME EESCEQQPQE EEEEEEDKEE EGDGADKTPK PPTDDPTSPT STPPEDQDST GKKPKAPAIR FLKRTLSNES EESVKSTSMP TDDPKTPTGS PATEVSTKWT HLTEFELKGL KALVEKLESL PENKKCVPEG IEDPQALLEG VKNVLKEHVD DDPTLAITGV PVVSWPKKTA KNRVVGRPKG KLGPASAVKL AANRTTAGAR RRRTRCRKCE ACLRTECGEC HFCKDMKKFG GPGRMKQSCI MRQCIAPVLP HTAVCLVCGE AGKEDTVEEE EGKFNLMLME CSICNEIIHP GCLKIKESEG VVNDELPNCW ECPKCNHAGK TGKQKRGPGF KYASNLPGSL LKEQKMNRDN KEGQEPAKRR SECEEAPRRR SDEHPKKVPA DGILRRKSDD VHLRRKRKYE KPQELSGRKR ASSLQTSPGS SSHLSPRPPL GSSLSPWWRS SLTYFQQQLK PGKEDKLFRK KRRSWKNAED RLSLANKPLR RFKQEPEDDL PEAPPKTRES DQSRSSSPTA GPSTEGAEGP EEKKKVKMRR KRRLVNKELS KELSKELNHE IQKTESTLAH ESQQPIKSEP ESENDEPKRP LSHCERPHRF SKGLNGTPRE LRHSLGPGLR SPPRVMSRPP PSASPPKCIQ MERHVIRPPP ISPPPDSLPL DDGAAHVMHR EVWMAVFSYL SHRDLCVCMR VCRTWNRWCC DKRLWTRIDL NRCKSITPLM LSGIIRRQPV SLDLSWTNIS KKQLSWLINR LPGLRDLVLS GCSWIAVSAL CSSSCPLLRT LDVQWVEGLK DAQMRDLLSP PTDNRPGQMD NRSKLRNIVE LRLAGLDITD VSLRLIIRHM PLLSKLQLSY CNHINDQSIN LLTAVGTTTR DSLTEVNLSD CNKVTDLCLS FFKRCGNICH IDLRYCKQVT KEGCEQFIAE MSVSVQFGQV EEKLLQKLS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: KDM2B (Lysine (K)-Specific Demethylase 2B (KDM2B))

Alternative Name: Kdm2b (KDM2B Products)

Synonyms: CXXC2 Protein, FBXL10 Protein, Fbl10 Protein, JHDM1B Protein, PCCX2 Protein, fbxl10 Protein, wu:fd13a09 Protein, zgc:85787 Protein, KDM2B Protein, Fbxl10 Protein, Cxxc2 Protein, E430001G17 Protein, Jhdm1b Protein, mKIAA3014 Protein, jhdm1b Protein, F6P23.18 Protein, F6P23_18 Protein, lysine demethylase 2B Protein, lysine (K)-specific demethylase 2Ba Protein, microRNA 7107 Protein, lysine (K)-specific demethylase 2B Protein, lysine (K)-specific demethylase 2B L homeolog Protein, F-box/RNI-like superfamily protein Protein, KDM2B Protein, kdm2ba Protein, Kdm2b Protein, MIR7107 Protein, kdm2b.L Protein, AT2G17020 Protein

Background: Lysine-specific demethylase 2B (EC 1.14.11.27) (F-box and leucine-rich repeat protein 10) (F-box protein FBL10) (F-box/LRR-repeat protein 10) (JmjC domain-containing histone demethylation protein 1B) ([Histone-H3]-lysine-36 demethylase 1B),FUNCTION: Histone demethylase that demethylates 'Lys-4' and 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' and dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. Preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which inhibits cell growth and proliferation (By similarity). May also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex (By similarity). {ECO:0000250|UniProtKB:Q8NHM5}.

Molecular Weight: 149.7 kDa

UniProt: Q6P1G2

Pathways: Tube Formation, Warburg Effect