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ARHGEF18 Protein (AA 1-1021) (His tag)

Crystallography grade ARHGEF18 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Catalog No. ABIN3135770
  • Target See all ARHGEF18 Proteins
    ARHGEF18 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 18 (ARHGEF18))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-1021
    Origin
    Mouse
    Source
    • 1
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This ARHGEF18 protein is labelled with His tag.
    Application
    ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
    Sequence
    MTISQKGGLQ PTPSPAGSGV RLGPIAGDMD EADSVFLKLK QTADDSLSLT SSNAESVFIE DPYIASLRCE IESDAHEFEA ESWSLSVDLA YAKKQKKEVV KRQDVLYELM QTEAHHVRTL KIMLKVYSRA LQEELQFSGQ AVSRLFPCAD DLLDMHSHFL ARLKERRQEF LEEGSDRNYV IQKIGDVLVQ QFSGETGERM KEKYAVFCSG HNDAVGQYKL LLQQSKKFQN LIKKIGNFSI VRRLGVQECI LLVTQRITKY PVLVERIIQN TEAGTEDYKD LSQALSLIKD IISQVDAKVS EYEKDQRLKE IAAKTDQKSS GKLKNGLTFR KEDMLQQRQL HLEGALCWKS TSGRLKDVLA VLLTDVLLLL QEKDQKYVFA SVDSKPPVIS LQKLIVREVA NEEKAMFLIS ASMQGPEMYE MYTSSKEDRN IWMAHIRRAV ESCPDEEEDV FSEAEEKKIA EARTMKLQEF QERLSLKDQL IAQSLLEKQQ IYLEMAQLSG LEESAQNRGL FRGGGDPSET LRGEQILRSA MSEIEGIQSL ICQRHLGSTS SQVEEGSVSA GLPRRAETFG GYDSVGSPSK GGSFKRKVSN SDLRPQDWQG PASSPDSRPC DNSAPSGCCE ESPQAVEMPS TESLPTVLEL ELVHRVQTLS QLLLSLQAVI AQQDSYVEMQ RTAIQEREKQ FRLQSTRGNL LLEQERQRNF EKQREERAGV EKLQSQLRQE QQRWERERAR QQQELELAGA RLQEREGEAR QMRQRLDQER TELERQRQAY QHDLERLREA QRAVDRERER LELLRRFKKQ NTVPGALPPE VLAEAQPASH PPSFNGDGLE GHSAPAKAPG TQGSAMLHGT GPDNVERPEV ARWDSAPPES RPAKSDVPIQ LLSATNQIQR QTAVQQQIPT KLAASTKGGK EKGSKSRGSQ RWESSASFDL KQQLLLSKFI GKDESASRNR RSLSPVLPAA HGSAPASDPC FPAPSPAPAA TPPEAFKFGG TSLPPVSPAS SLPTTPLATT DEVSKEDVIF F
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Arhgef18 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product ARHGEF18 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    ARHGEF18 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 18 (ARHGEF18))
    Alternative Name
    Arhgef18 (ARHGEF18 Products)
    Synonyms
    arhgef18 Protein, wu:fc21e04 Protein, wu:fj05f11 Protein, zgc:85686 Protein, P114-RhoGEF Protein, AI467246 Protein, D030053O22Rik Protein, rho/rac guanine nucleotide exchange factor (GEF) 18a Protein, Rho/Rac guanine nucleotide exchange factor (GEF) 18 Protein, Rho/Rac guanine nucleotide exchange factor 18 Protein, rho guanine nucleotide exchange factor 18 Protein, rho/rac guanine nucleotide exchange factor (GEF) 18 Protein, arhgef18a Protein, ARHGEF18 Protein, LOC100027283 Protein, Arhgef18 Protein
    Background
    Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. Also act as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production. Required for EPB41L4B-mediated regulation of the circumferential actomyosin belt in epithelial cells. {ECO:0000250|UniProtKB:Q6ZSZ5}.
    Molecular Weight
    115.3 kDa Including tag.
    UniProt
    Q6P9R4
    Pathways
    Neurotrophin Signaling Pathway
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