SARM1 Protein (AA 28-724) (His tag)

Catalog No. ABIN3135795

Product Details

Target: SARM1 (Sterile alpha and TIR Motif Containing 1 (SARM1))

Protein Type: Recombinant

Protein Characteristics: AA 28-724

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This SARM1 protein is labelled with His tag.

Application: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: LTVPGPDRSG GASPWWAAGG RGSREVSPGV GTEVQGALER SLPELQQALS ELKQASAARA VGAGLAEVFQ LVEEAWLLPA VGREVAQGLC DAIRLDGGLD LLLRLLQAPE LETRVQAARL LEQILVAENR DRVARIGLGV ILNLAKEREP VELARSVAGI LEHMFKHSEE TCQRLVAAGG LDAVLYWCRR TDPALLRHCA LALANCALHG GQTVQRCMVE KRAAEWLFPL AFSKEDELLR LHACLAVAVL ATNKEVEREV EHSGTLALVE PLVASLDPGR FARCLVDASD TSQGRGPDDL QSLVLLLDSS RLEAQCIGAF YLCAEAAIKS LQGKTKVFSD IGAIQSLKRL VSYSTNGTTS ALAKRALRLL GEEVPRRILP CVASWKEAEV QTWLQQIGFS QYCENFREQQ VDGDLLLRLT DEELQTDLGM KSSITRKRFF RELTELKTFA SYATCDRSNL ADWLGSLDPR FRQYTYGLVS CGLDRSLLHR VSEQQLLEDC GIRLGVHRTR ILSAAREMLH SPLPCTGGKL SGDTPDVFIS YRRNSGSQLA SLLKVHLQLH GFSVFIDVEK LEAGKFEDKL IQSVIAARNF VLVLSAGALD KCMQDHDCKD WVHKEIVTAL SCGKNIVPII DGFEWPEPQA LPEDMQAVLT FNGIKWSHEY QEATIEKIIR FLQGRPSQDS SAGSDTSLEG ATPMGLP
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Sarm1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SARM1 (Sterile alpha and TIR Motif Containing 1 (SARM1))

Alternative Name: Sarm1 (SARM1 Products)

Synonyms: A830091I15Rik Protein, C78606 Protein, MyD885 Protein, Sarm Protein, MyD88-5 Protein, SAMD2 Protein, SARM Protein, sterile alpha and TIR motif containing 1 Protein, sterile alpha and HEAT/Armadillo motif containing 1 Protein, SARM1 Protein, Sarm1 Protein, sarm1 Protein

Background: Negative regulator of MYD88- and TRIF-dependent toll-like receptor signaling pathway which plays a pivotal role in activating axonal degeneration following injury. Promotes Wallerian degeneration an injury-induced axonal death pathway which involves degeneration of an axon distal to the injury site. Can activate neuronal death in response to stress. Regulates dendritic arborization through the MAPK4-JNK pathway. Involved in innate immune response. Inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38. Can restrict West Nile virus (WNV) pathogenesis. {ECO:0000269|PubMed:17724133, ECO:0000269|PubMed:19587044, ECO:0000269|PubMed:21555464, ECO:0000269|PubMed:22678360}.

Molecular Weight: 77.5 kDa Including tag.

UniProt: Q6PDS3

Pathways: TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades