EFR3B Protein (AA 1-817) (His tag)
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- Target See all EFR3B products
- EFR3B (EFR3 Homolog B (EFR3B))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-817
- Origin
- Mouse
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Source
- Insect Cells
- Purification tag / Conjugate
- This EFR3B protein is labelled with His tag.
- Application
- Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MYGVCGCCGA LRPRYKRLVD NIFPEDPEDG LVKTNMEKLT FYALSAPEKL DRIGAYLSER LIRDVGRHRY GYVCIAMEAL DQLLMACHCQ SINLFVESFL KMVAKLLESE KPNLQILGTN SFVKFANIEE DTPSYHRSYD FFVSRFSEMC HSSHDDLEIK TKIRMSGIKG LQGVVRKTVN DELQANIWDP QHMDKIVPSL LFNLQHVEEA ESRSPSPLQA PEKEKENPAE LAERCLRELL GRAAFGNIKN AIKPVLIHLD NHSLWEPKVF ATRCFKIIMY SIQPQHSHLV IQQLLSHLDA NSRSAATVRA GIVEVLSEAA IIAATGSVGP TVLEMFNTLL RQLRLSIDYA LTGSYDGAVS LGSKIIKEHE ECMFQEAVIK TIGSFASTLP TYQRSEVILF IMSKVPLPSV HHPVETGRTG ENRNRLTQIM LLKSLLQVST GFQCNNMMSA LPSNFLDRLL STALMEDAEI RLFVLEILIS FIDRHGNRHK FSTISTLGDI SVLKLKVDKC SRQDTVFMKK HSQQLYRHIY LSCKEETNIQ KHYEALYGLL ALISIELANE EVVVDLIRLV LAVQDVAQVN EENLPTYNRC ALYALGAAYL NLISQLTTVP AFCQHIHEVI ETRKKEAPYM LPEDVFVEKP RLSQNLDGVV IEFLFRQSKI SEVLGGSGYN SDRLCLPYIP QLTDEDRLSK RKSIGETISL QVEVESRNSP EKEERVPAEE ITYETLKKAI VDSVAVEEQE RERQRQVVEK FQKAPFEEIA AHCGARASLL QSKLNQIFEI TIRPPPSPSG TISAAYGQPQ NHSIPVYEMK FPDLCVY
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Efr3b Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- EFR3B (EFR3 Homolog B (EFR3B))
- Alternative Name
- Efr3b (EFR3B Products)
- Synonyms
- KIAA0953 Protein, AI852640 Protein, C030014M07Rik Protein, mKIAA0953 Protein, efr3b Protein, si:ch211-203k16.1 Protein, si:ch211-215m21.18 Protein, si:dkeyp-70e6.6 Protein, EFR3 homolog B Protein, EFR3 homolog Bb (S. cerevisiae) Protein, EFR3B Protein, Efr3b Protein, efr3bb Protein
- Background
- Component of a complex required to localize phosphatidylinositol 4-kinase (PI4K) to the plasma membrane. The complex acts as a regulator of phosphatidylinositol 4-phosphate (PtdIns(4)P) synthesis. In the complex, EFR3B probably acts as the membrane-anchoring component. Also involved in responsiveness to G-protein-coupled receptors, it is however unclear whether this role is direct or indirect. {ECO:0000250|UniProtKB:Q9Y2G0}.
- Molecular Weight
- 93.4 kDa Including tag.
- UniProt
- Q6ZQ18
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