LARP1 Protein (AA 2-1072) (His tag)

Catalog No. ABIN3135877

Product Details

Target: LARP1 (La Ribonucleoprotein Domain Family, Member 1 (LARP1))

Protein Type: Recombinant

Protein Characteristics: AA 2-1072

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This LARP1 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: ATQVEPLLPA GAPLLQAEEH GLARKKPAPD AQAESGPGDG GGEPDGGVRR PRPACARPGR DGAERESPRP PAAAEAPAGS DGEDGGRRDF VEAPPPKVNP WTKHAPPPAA VNGQPPPEPS APAKVVRAAA PKPRKGSKVG DFGDAVNWPT PGEIAHKSVQ PQSHKPQPAR KLPPKKDMKE QEKGDGSDSK ESPKTKSDES GEEKNGDEDC QRGGQKKKGS KHKWVPLQID MKPEVPREKL ASRPTRPQEP RHTPAVRGEM KGSEPATYMP VSVAPPTPAW QPETKVEPAW HDQDETSSVK SDGAGGARAS FRGRGRGRGR GRGRGRGGTR THFDYQFGYR KFDGTEGPRT HKYMNNITYY FDNVSSNEIY SMDQELLKDY IKRQIEYYFS VDNLERDFFL RRKMDADGFL PITLIASFHR VQALTTDISL IFAALKDSKV VEMVEEKVRR REEPEKWPLP GPPIVDYSQT DFSQLLNCPE FVPRQHYQKE TESAPGSPRA VTPVPTKTEE VSNLKTLPKG LSASLPDLDS ESWIEVKKRP RPSPARPKKP EEPRFSHPTA LPQQLPSQQL MSKDQDEQEE LDFLFDEEME QMDGRKNTFT AWSEEDSDYE IDDRDVNKIL IVTQTPPYMR RHPGGDRTGN HTSRAKMSAE LAKVINDGLF YYEQDLWTEK FEPEYSQIKQ EVENFKKVNM ISREQFDTLT PEPPVDPNQE VPPGPPRFQQ VPTDALANKL FGAPEPSTIA RSLPTTVPES PNYRNARTPR TPRTPQLKDS SQTPRFYPVV KEGRTLDAKM PRKRKTRHSS NPPLESHVGW VMDSREHRPR TASISSSPSE GTPAVGSYGC TPQSLPKFQH PSHELLKENG FTQHVYHKYR RRCLNERKRL GIGQSQEMNT LFRFWSFFLR DHFNKKMYEE FKQLALEDAK EGYRYGLECL FRYYSYGLEK KFRLDIFKDF QEETVKDYEA GQLYGLEKFW AFLKYSKAKN LDIDPKLQEY LGKFRRLEDF RVDPPMGEEG NHKRHPVVAG GSGEGRKRCP SQSSSRPATG ISQPPTTPTG QATREDAKWT SQHSDTLTLR K
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Larp1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: LARP1 (La Ribonucleoprotein Domain Family, Member 1 (LARP1))

Alternative Name: Larp1 (LARP1 Products)

Synonyms: RGD1306683 Protein, wu:fb92d03 Protein, wu:fd15e07 Protein, 3110040D16RIK Protein, MGC98945 Protein, LARP Protein, 1810024J12Rik Protein, 3110040D16Rik Protein, Larp Protein, mKIAA0731 Protein, La ribonucleoprotein domain family, member 1 Protein, La ribonucleoprotein domain family member 1 Protein, La ribonucleoprotein domain family member 1 L homeolog Protein, Larp1 Protein, larp1 Protein, LARP1 Protein, larp1.L Protein

Background: RNA-binding protein that promotes translation of specific classes of mRNAs downstream of the mTORC1 complex. Associates with the mRNA 5'cap in an MTOR-dependent manner and associates with mRNAs containing a 5' terminal oligopyrimidine (5'TOP) motif, which is present in mRNAs encoding for ribosomal proteins and several components of the translation machinery. Associates with actively translating ribosomes via interaction with PABPC1/PABP and stimulates translation of mRNAs containing a 5'TOP, thereby regulating cell growth and proliferation (By similarity). {ECO:0000250}.

Molecular Weight: 121.9 kDa Including tag.

UniProt: Q6ZQ58

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection