SYNM Protein (AA 1-1561) (Strep Tag)

Catalog No. ABIN3135892

Product Details

Target: SYNM (Synemin, Intermediate Filament Protein (SYNM))

Protein Type: Recombinant

Protein Characteristics: AA 1-1561

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This SYNM protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MLSWRLQTGS EKAELQELNA RLYDYVCRVR ELERENLLLE EELRSRLSRE DRWAEDQALY AEEARSLRQQ LDELNWSTAL AEGERDALRR ELLELQREGV EAGTARSRLD AELGAQRREL EEALGARAAL EALLGRLETE RRDLDAAHER QVRDLRARAA SLTMHFRARA TSPAAPPPRL RDVHDSYALL VAESWRESVQ LYEDEVRELE QALRRGQESR LQAEDEARLC AQEADALRNQ ALELEQLRAR LEDELLRMRE EYGMQAEERQ RVIDSLEDEK EALTLAMADR LRDYQELLQV KTGLSLEVAT YRALLEGESN PEILIWTENI ENVPQEPRNT SYRYTNSVLQ RKNEKNLFPR RKTPWAAVNH SSASYSNWPG HLDSQTTTAV GSAARRGLLT SRHSSSATTS GQQKPLEKTI SSRANLRPVT PTHGFLRNTD AQMKTLPHRS KVEGTGDTHA RRATESVITR ESYRGHQGHV AAGAVSSTPS NERTVILGKK LEAQATKEQE RDRSGVIRIK PEEKMFDSKE KASEERNLRW EELTKLDRDA RKRESRHLRD EAREKEALKE RSVKEREVPI SLEVSRGSRA EVSTIHLQSP GRKDVSHSGG REAETKETRF RLDTQDTASS LQSDSTTETI AESIVTTILK QFTQSPGAEE EATSFPDTKV TYVDRKEFPG DGKTKTEIVV ESKLTDVVDV SDEAGLDYLL SKDVKEVGLK GKSTETMIGE MINLGLKGRE GRAKVVNVEI VEEPMSYIGG GKIDFSTPFQ VEEVDDVSPS PKGFVEEEDG EGETHMAFSM RPHQTKQPQG TIPHVEEVTE AGDSEGEQSY FVSTPDEYPG GHDREDDGSV YGQIHIEEES TIRYSWQDEI AQGTWRRKMR GDVGGEKPVK VLEVPALSLG GAIGSAHLKE EASGELRAEP TVIEKEIKIP HEFHTSIKGV FSSEPRHQLV EVIGQLEETL PERMKEELSA LTRQSQGESG SVSVDVKKVQ SAAGGSVTLM AEVNLSQTVD ADQLDLEQLS RDEAGEIERA VESVVRESLA KRSSPVPRSP DREDGEEVPA GGILFKRWAT RELYSPSGER DDAGQVSPSS DQRVTQGPVS ATVEVTSPTG FVQSHVLEDV SQSVRHVKLG PTEMWRTEQV TFGGPTAQVV EVSGDFSEAV SSEGASRSVR HITLGPHQSQ VSTEVIFRGS VPTWQETGDT EKPGPVVLSV GADISGSGRM PGSERSHTEK EIRFQGPVSG TAQVGGNFAT EESVGSQTFV RSLQLGPKEG FREEIQFIAP IPDKVGWGEE DSEHTKVSLE RATSIQRIDI VPQRYLASKQ MAPQTLEFRD SEDMVMVEGS AGTIQATHNF TSDREILQNK ENTFQRVISG SPPDSVGDTG AEVTANVSRS FRHIQIGPTE EEPSEYFVTG RPVSKTFVLD GSVASPGLVG GADGGSTPCR IALGPKETSF TFQMDLSDTR AIRSWTRDTG SEVEAHGVSH RGGWRIAHSR DERVASTGSG ASPGDAHQAP GEKGTEQAGF DKTVQLQRMV DQRSVASDEK KVALLYLDNE EEEEEEGEGW F
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SYNM (Synemin, Intermediate Filament Protein (SYNM))

Alternative Name: Synm (SYNM Products)

Synonyms: DMN Protein, dmn Protein, si:dkey-25f3.3 Protein, SYN Protein, 4930412K21Rik Protein, AI852401 Protein, Dmn Protein, E130104F11 Protein, Syn Protein, Synemin Protein, synemin Protein, synemin, intermediate filament protein Protein, SYNM Protein, synm Protein, Synm Protein

Background: Synemin (Desmuslin),FUNCTION: Type-VI intermediate filament (IF) which plays an important cytoskeletal role within the muscle cell cytoskeleton. It forms heteromeric IFs with desmin and/or vimentin, and via its interaction with cytoskeletal proteins alpha-dystrobrevin, dystrophin, talin-1, utrophin and vinculin, is able to link these heteromeric IFs to adherens-type junctions, such as to the costameres, neuromuscular junctions, and myotendinous junctions within striated muscle cells (By similarity). {ECO:0000250, ECO:0000269|PubMed:15265691}.

Molecular Weight: 173.2 kDa

UniProt: Q70IV5