SPAG5 Protein (AA 1-1165) (Strep Tag)

Catalog No. ABIN3135916

Product Details

Target: SPAG5 (Sperm Associated Antigen 5 (SPAG5))

Protein Type: Recombinant

Protein Characteristics: AA 1-1165

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This SPAG5 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MWRVKTLNLG LSPSPQKGKP AMSTPLRELK LQPEALADSG KGPSMISALT PYLCRLELKE RCNNSSPVDF INTENNFLSE QFSHPSTHIE ACQRESDPTP ESNSLFHTLE EAIETVDDFV VDPRDDSIVE SMVLLPFSLG QQQDLMLQAH LDTTAERTKS SLNESLGLED LVGKEVAPCV EDSLTEIVAI RPEQPTFQDP PLGPSDTEDA PVDLVPSENV LNFSLARLSP SAVLAQDFSV DHVDPGEETV ENRVLQEMET SFPTFPEEAE LGDQAPAANA EAVSPLYLTS SLVEMGPREA PGPTVEDASR IPGLESETWM SPLAWLEKGV NTSVMLQNLR QSLSFSSVLQ DAAVGNTPLA TCSVGTSFTP PAPLEVGTKD STSETERLLL GCRPPDLATL SRHDLEENLL NSLVLLEVLS HQLQAWKSQL TVPHREARDS STQTDSSPCG VTKTPKHLQD SKEIRQALLQ ARNVMQSWGL VSGDLLSLLH LSLTHVQEGR VTVSQESQRS KTLVSSCSRV LKKLKAKLQS LKTECEEARH SKEMALKGKA AAEAVLEAFR AHASQRISQL EQGLTSMQEF RGLLQEAQTQ LIGLHTEQKE LAQQTVSLSS ALQQDWTSVQ LNYGIWAALL SWSRELTKKL TAKSRQALQE RDAAIEEKKQ VVKEVEQVSA HLEDCKGQIE QLKLENSRLT ADLSAQLQIL TSTESQLKEV RSQHSRCVQD LAVKDELLCQ LTQSNKEQAT QWQKEEMELK HIQAELLQQQ AVLAKEVQDL RETVEFIDEE SQVAHRELGQ IESQLKVTLE LLRERSLQCE TLRDTVDSLR AELASTEAKH EKQALEKTHQ HSQELRLLAE QLQSLTLFLQ AKLKENKAES EIILPSTGSA PAQEHPLSND SSISEQTPTA AVDEVPEPAP VPLLGSVKSA FTRVASMASF QPTETPDLEK SLAEMSTVLQ ELKSLCSLLQ ESKEEATGVL QREICELHSR LQAQEEEHQE ALKAKEADME KLNQALCLLR KNEKELLEVI QKQNEKILGQ IDKSGQLINL REEVTQLTQS LRRAETETKV LQEALEGQLD PSCQLMATNW IQEKVFLSQE VSKLRVMFLE MKTEKEQLMD KYLSHRHILE ENLRRSDTEL KKLDDTIQHV YETLLSIPET MKSCKELQGL LEFLS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SPAG5 (Sperm Associated Antigen 5 (SPAG5))

Alternative Name: Spag5 (SPAG5 Products)

Synonyms: SPAG5 Protein, DEEPEST Protein, MAP126 Protein, hMAP126 Protein, AI874642 Protein, D11Bhm180e Protein, Deepest Protein, Mastrin Protein, S17 Protein, sperm associated antigen 5 Protein, sperm-associated antigen 5 Protein, SPAG5 Protein, LOC739263 Protein, Spag5 Protein

Background: Sperm-associated antigen 5 (Mastrin) (Mitotic spindle-associated protein p126) (MAP126),FUNCTION: Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase. Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. In complex with SKAP, promotes stable microtubule-kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes. Involved in centriole duplication. Required for CDK5RAP22, CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2. In non-mitotic cells, upon stress induction, inhibits mammalian target of rapamycin complex 1 (mTORC1) association and recruits the mTORC1 component RPTOR to stress granules (SGs), thereby preventing mTORC1 hyperactivation-induced apoptosis. May enhance GSK3B-mediated phosphorylation of other substrates, such as MAPT/TAU (By similarity). {ECO:0000250|UniProtKB:Q96R06}.

Molecular Weight: 130.0 kDa

UniProt: Q7TME2

Pathways: M Phase