FBXO11 Protein (AA 1-930) (Strep Tag)

Catalog No. ABIN3135931

Product Details

Target: FBXO11 (F-Box Protein 11 (FBXO11))

Protein Type: Recombinant

Protein Characteristics: AA 1-930

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This FBXO11 protein is labelled with Strep Tag.

Application: SDS-PAGE (SDS), ELISA, Western Blotting (WB)

Sequence: MNSVRAANRR PRRVSRPRPV QQQQQQPPQQ PPPQPPQQQP PPQPPQQPPQ QQPPPPPQQQ PPPPPPPPPP PPQDRNNAGE RDDVPADMVA EESGPGAQNS PYQLRRKTLL PKRTACPTKS SMEGASTSTT ENFGHRAKRA RVSGKSQDLS AAPAEQYLQE KLPDEVVLKI FSYLLEQDLC RAACVCKRFS ELANDPILWK RLYMEVFEYT RPMMHPEPGK FYQINPEEYE HPNPWKESFQ QLYKGAHVKP GFAEHFYSNP ARYKGRENML YYDTIEDALG GVQEAHFDGL IFVHSGIYTD EWIYIESPIT MIGAAPGKVA DKVIIENTRD STFVFMEGSE DAYVGYMTIR FNPDDKSAQH HNAHHCLEIT VNCSPIIDHC IIRSTCTVGS AVCVSGQGAC PTIKHCNISD CENVGLYITD HAQGIYEDNE ISNNALAGIW VKNHGNPIIR RNHIHHGRDV GVFTFDHGMG YFESCNIHRN RIAGFEVKAY ANPTVVRCEI HHGQTGGIYV HEKGRGQFIE NKIYANNFAG VWITSNSDPT IRGNSIFNGN QGGVYIFGDG RGLIEGNDIY GNALAGIQIR TNSCPIVRHN KIHDGQHGGI YVHEKGQGVI EENEVYSNTL AGVWVTTGST PVLRRNRIHS GKQVGVYFYD NGHGVLEDND IYNHMYSGVQ IRTGSNPKIR RNKIWGGQNG GILVYNSGLG CIEDNEIFDN AMAGVWIKTD SNPTLRRNKI HDGRDGGICI FNGGRGLLEE NDIFRNAQAG VLISTNSHPV LRKNRIFDGF AAGIEITNHA TATLEGNQIF NNRFGGLFLA SGVNVTMKDN KIMNNQDAIE KAVSRGQCLY KISSYTSYPM HDFYRCHTCN TTDRNAICVN CIKKCHQGHD VEFIRHDRFF CDCGAGTLSN PCTLAGEPTH DTDTLYDSAP PIESNTLQHN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: FBXO11 (F-Box Protein 11 (FBXO11))

Alternative Name: Fbxo11 (FBXO11 Products)

Synonyms: FBX11 Protein, PRMT9 Protein, UBR6 Protein, VIT1 Protein, C80048 Protein, Jf Protein, Vit1 Protein, fbxo11 Protein, MGC84634 Protein, FBXO11 Protein, im:6906507 Protein, wu:fj83f06 Protein, zgc:153171 Protein, Fbxo11 Protein, DKFZp459P2410 Protein, F-box protein 11 Protein, F-box protein 11 L homeolog Protein, F-box protein 11a Protein, F-box only protein 11 Protein, hypothetical protein Protein, FBXO11 Protein, Fbxo11 Protein, fbxo11.L Protein, fbxo11a Protein, CpipJ_CPIJ009919 Protein, CpipJ_CPIJ017549 Protein, Bm1_52225 Protein, LOAG_16571 Protein, fbxo11 Protein, LOC100115478 Protein

Background: F-box only protein 11,FUNCTION: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as DTL/CDT2, BCL6 and PRDM1/BLIMP1. The SCF(FBXO11) complex mediates ubiquitination and degradation of BCL6, thereby playing a role in the germinal center B-cells terminal differentiation toward memory B-cells and plasma cells. The SCF(FBXO11) complex also mediates ubiquitination and degradation of DTL, an important step for the regulation of TGF-beta signaling, cell migration and the timing of the cell-cycle progression and exit. Binds to and neddylates phosphorylated p53/TP53, inhibiting its transcriptional activity. Plays a role in the regulatiom of erythropoiesis but not myelopoiesis or megakaryopoiesis. Mechanistically, activates erythroid genes by mediating the degradation of BAHD1, a heterochromatin-associated protein that recruits corepressors to H3K27me3 marks. Participates in macrophage cell death and inflammation in response to bacterial toxins by regulating the expression of complement 5a receptor 1/C5AR1 and IL-1beta. Acts as a critical regulator to determine the level of MHC-II by mediating the recognition of degron at the P/S/T domain of CIITA leading to its ubiquitination and subsequent degradation via the proteasome. Participates in the antiviral repsonse by initiating the activation of TBK1-IRF3-IFN-I axis. Mediates the 'Lys-63'-linked ubiquitination of TRAF3 to strengthen the interaction between TRAF3 and TBK1. {ECO:0000250|UniProtKB:Q86XK2}.

Molecular Weight: 103.7 kDa

UniProt: Q7TPD1

Pathways: Sensory Perception of Sound