PRRC2B Protein (AA 1-1486) (Strep Tag)

Catalog No. ABIN3135934

Product Details

Target: PRRC2B (Proline-Rich Coiled-Coil 2B (PRRC2B))

Protein Type: Recombinant

Protein Characteristics: AA 1-1486

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This PRRC2B protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MSDRLGQITQ GKDGKSKYST LSLFDKYKGR SVGAVRSSVI PRHGLQSLGK VATARRMPPP ANLPSLKSEN KGNDPNIVIV PKDGTGWANK QDQQDPKSSS VTASQPPESQ PQPGLQKSVS NLQKPTQSIS QENTNSVPGG PKSWAQLSGK PVGHEGGLRG SSRLLSFSPE EFPTLKAAGG QDKAGKEKGA LDLSYGPGPS LRPQNVTSWR EGGGRNIISA ASLSASPTEL GSRNASGADG APSLACTSDS KEPSLRPAQP SRRGASQFMG HGYQPPTYHD MLPAFMCSPQ SSENQTTVER SSFPLPQLRL EPRVPFRQFQ MNDQDGKERP GVARPVRPLR QLVERAPRPT IINAENLKGL DDLDTDADDG WAGLHEEVDY SEKLKFSDDE DEEDVVKDGR SKWNNWDPRR QRALSLSSAD STDAKRTQEE GKDWSGTAGG SRVIRKVPEP QPPSRKLHSW ASGPDYQKPT MGSMFRQHSA EDKEDKPPPR QKFIQSEMSE AVERARKRRE EEERRAREER LAACAAKLKQ LDQKCRQAQK ANETPKPVEK EVPRSPGIEK VSPPENGPVV RKGSPEFPVQ EAPTMFLEET PATSPTVAQS NSSSSSSSSS SIEEEVRESG SPAQEFSKYQ KSLPPRFQRQ QQQQQQQQQQ QQQQEQLYKM QHWQPVYPPP SHPQRTFYPH HPQMLGFDPR WMMMPSYMDP RITPTRTPVD FYPSALHPSG LMKPMMPQES LSGTGCRSED QNCVPSLQER KVTALDPAPV WSPEGYMALQ NKGYSLPHPK SADTLAMGMH VRSPDEALPG GLGSHSPYAL ERTTHASSDG PETPSKKSER EVSLPTQRAS EQEEARKQFD LGYGNALIDN CASSPGEENE ASSVVGEGFI EVLTKKQRRL LEEERRKKEQ AAQVPVKGRG LSSRIPPRFA KKQNGLCLEQ DVTVPGSSLG TEIWENSSQA LPVQGAASDS WRTAVTAFSS TEPGTSEQGF KSSQGDSGVD LSAESRESSA TSSQRSSPYG TLKPEEISGP GLAESKADSH KDQAQKQAEH KDSEQGSAQS KEHRPGPIGN ERSLKNRKGS EGAERLPGAV VPPVNGVEIH VDSVLPVPPI EFGVSPKDSD FSLPPGSVSG PVGNPVAKLQ DVLASNAGLT QSIPILRRDH HMQRAIGLSP MSFPTADLTL KMESARKAWE NSPSLPEQSS PGGAGSGIQP PSSVGASNGV NYSSFGGVSM PPMPVASVAP SASIPGSHLP PLYLDGHVFA SQPRLVPQTI PQQQSYQQAA TAQQIPISLH TSLQAQAQLG LRGGLPVSQS QEIFSSLQPF RSQVYMHPSL SPPSTMILSG GTALKPPYSA FPGIQPLEMV KPQSGSPYQP MSGNQALVYE GQLGQAAGLG TSQMLDSQLP QLTMPLPRYG SGQQPLILPQ SIQLPPGQSL SVGAPRRVPP PGSQPPVLNT SRESAPMELK GFHFADSKQN VPTGGSAPSP QAYRQSEWMK NPAWEP
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PRRC2B (Proline-Rich Coiled-Coil 2B (PRRC2B))

Alternative Name: Prrc2b (PRRC2B Products)

Synonyms: BAT2L Protein, BAT2L1 Protein, KIAA0515 Protein, LQFBS-1 Protein, 5830434P21Rik Protein, AI173903 Protein, Bat2l Protein, Bat2l1 Protein, D430039P21 Protein, mKIAA0515 Protein, proline rich coiled-coil 2B Protein, proline-rich coiled-coil 2B Protein, PRRC2B Protein, Prrc2b Protein

Background: Protein PRRC2B (HLA-B-associated transcript 2-like 1) (Proline-rich coiled-coil protein 2B)

Molecular Weight: 160.9 kDa

UniProt: Q7TPM1

Pathways: SARS-CoV-2 Protein Interactome